ایجاد و تعیین ویژگی‌های یک رده سلولی جدید از هموسیت‌های لارو زنبور برگخوار رز Arge ochropus (Hymenoptera; Argidae)

نوع مقاله: مقاله کامل، انگلیسی

نویسندگان

1 دانشگاه گیلان

2 گروه گیاه پزشکی دانشگاه گیلان

3 گیاه پزشکی دانشگاه گیلان

4 دانشگاه علوم پزشکی اصفهان

چکیده

یک رده­ی سلولی جدید از سلول­های خونی لارو زنبور برگ‌خوار رز، Arge ochropusایجاد شد. این رده سلولی براساس نام Arge ochropus University of Guilan، AOU-G نام­گذاری شد. این رده­ی سلولی از سلول­های خونی لارو زنبور برگ‌خوار رز، به منظور ارزیابی خصوصیات و حساسیت به اکدایزون مورد بررسی قرار گرفت. ویژگی‌های شکل‌شناسی سلول‌های آن با استفاده از میکروسکوپ‌های نوری بررسی شد. از نظر ریخت شناسی در کشت اولیه سلول­های خونی، چهار نوع سلول مشهود بود که به­طور عمده شامل پروهموسیت‌ها، پلاسماتوسیت‌ها، گرانولوسیت‌ها و انوسیتوئیدها بود. زمان مضاعف شدن جمعیت این رده سلولی در بیستمین کشت مجدد در دمای 28 درجه­ی سلسیوس روی محیط کشت Ex-cell 400 و گریس به­ترتیب 3 و 5 روز بود. مطالعات بیوشیمیایی روی کشت اولیه ناشی از سلول­های خونی در محیط کشت Ex-cell 400 و گریس، نمایانگر تغییرات قابل ملاحظه­ای در برخی از ترکیبات نظیر گلوکز، پروتئین، اوره و کلسترول در محیط کشت بود. به غیر از اوره که افزایش معنی­داری را در محیط کشت نشان داد، بقیه ترکیبات اندازه­گیری شده کاهش چشمگیری پیدا نمودند. ردة سلولی AOU-G را به عنوان یک ردة سلولی جدید معرفی می­کنیم که می­تواند در مطالعات مربوط به زیست‌شناسی، ایمونولوژی، غدد درون ریز وسم­شناسی بسیار مفید باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Establishment and characterizations of a new cell line from larval hemocytes of rose sawfly Arge ochropus (Hymenoptera; Argidae)

نویسندگان [English]

  • Bita Valizade 2
  • Roya Khosravi 3
  • Rasoul Salehi 4
2 Plant Protection, University of Guilan
3 Plant Protection, University of Guilan
4 University of Isfahan
چکیده [English]

We established and characterized a hymenopteran cell line from Arge ochropus for the first time. The established cell line was named AOU-G, standing for Arge ochropus University of Guilan. This cell line was derived from larval hemocytes of the rose sawfly whose characteristics and susceptibility to 20-hydroxyecdysone have been verified. We also noticed four types of hemocytes such as prohemocytes, plasmatocytes, granulocytes and oenocytes. The population doubling time of 20th passage of this new cell line in EX-cell or Grace medium at 28˚C was 3 and 5 days, respectively. Biochemical studies in rose sawfly hemocytes showed considerable changes in some component: protein, glucose, cholesterol and urea. Except urea, all components were significantly reduced. We suggest that the AOU-G cell line as a productive new cell line that could be very useful in studies concerning bio- insecticides, immunology, endocrinology and toxicology. 

کلیدواژه‌ها [English]

  • Arge ochropus
  • Baculovirus
  • Cell culture
  • Ecdysone
  • Hemocytes

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