TY - JOUR ID - 109506 TI - استراتژی سرما سختی لاروهای بید چغندر قند Scrobipalpa ocellatella (Lepidoptera: Gelechiidae) جمع آوری شده از مزرعه JO - نامه انجمن حشره شناسی ایران JA - JESI LA - fa SN - 0259-9996 AU - گنجی, زهرا AU - محرمی پور, سعید AD - پردیس کشاورزی و منابع طبیعی دانشگاه تهران AD - گروه حشره شناسی دانشکده کشاورزی دانشگاه تربیت مدرس Y1 - 2017 PY - 2017 VL - 36 IS - 4 SP - 287 EP - 296 KW - Cold hardiness KW - inoculative freezing point KW - lower lethal temperature KW - beet mothlarvae KW - supercooling point KW - Iran DO - N2 - The beet moth, Scrobipalpa ocellatella is recognized as a widespread agricultural pest. Cold hardiness strategy of the beet moth larvae was investigated through monitoring seasonal changes at supercooling points and lower lethal temperatures. Furthermore, the role of microhabitat in winter survival was studied. The mean SCPs of the last instar larvae was not significantly different from November 2010 to April 2011. Mean inoculative freezing point (-8.0 ± 1.44 °C) of the last instar larvae was significantly higher than mean SCP (-14.9 ± 0.93 °C). The cold hardiness of the pest shows seasonal fluctuation in response to reduction of air temperature. A 50% mortality (LT50) occurred at -11 ºC in November and -14 ºC in January and reduced to -18 ºC in February and finally increased to -14.5 ºC in April. Glycerol, sorbitol, trehalose, and myo-inositol were identified components in whole body extracts of S. ocellatella larvae. However, total cryoprotectants could not have significant effects on the cold tolerance. Larvae of S. ocellatella could tolerate subzero temperatures near their SCPs. Our findings show that beet moth larvae utilize moderately chill tolerance strategy during winter. UR - https://jesi.areeo.ac.ir/article_109506.html L1 - https://jesi.areeo.ac.ir/article_109506_1468a0c230c0c73065106c031c9e188d.pdf ER -