تأثیر گیاه توتون تراریخته، Nicotiana tabacum حاوی ژن بازدارنده فعالیت ریبوزوم جدا شده از پیاز گیاه زنبق هلندی، Iris hollandica روی پارامترهای بیولوژیکی(Myzus nicotianae (Hem.: Aphididea و (Spodoptera exigua (Lep.: Nocuidae

نویسنده

گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ولی‌عصر(عج) رفسنجان، ایران.

چکیده

استفاده از گیاهان زراعی اصلاح شده با استفاده از انتقال ژن بین گونه‌های مختلف گیاهی نقش مهمی‌در کنترل آفات گیاهی داشته است. در این پژوهش، خواص حشره‌کشی دو لاین از گیاهان تراریخته شاملIRIP  (حاوی ژن بازدارنده ریبوزوم نوع 1) و IRA (حاوی ژن بازدارنده ریبوزوم نوع 2) علیه کرم برگخوار چغندرقندSpodoptera exigua Hübner (Lepidoptera: Noctuidae) و شته توتون،Blackman (Hemiptera: Aphididae)  Myzus nicotianae بررسی شده است. تغذیه از گیاه توتون تراریخته IRA، باعث افزایش مرگ­ومیر و کاهش تولید مثل در شته توتون شد. هم‌چنین طول دوره تولیدمثلی شته‌های پرورش یافته روی گیاه IRA در مقایسه با تیمار شاهد و IRIP کاهش یافت. اما پارامترهای تولیدمثلی شته‌های تیمار شده باIRIP  تفاوت معنی­داری با شاهد نداشتند. تغذیه لاروهای سن دو exigua . Sاز گیاه تراریخته IRA، باعث مرگ­ومیر حدود 3/33 درصد شد. به­علاوه وزن لاروها و شفیره‌ها و درصد خروج حشرات کامل S. exigua نیز در تیمار  IRA نسبت به شاهد و تیمار گیاهان تراریخته  IRIPکاهش معنی­داری نشان دادند. نتایج حاکی از این است که پروتئین‌های بازدارنده فعالیت ریبوزوم نوع دوم دارای فعالیت حشره‌کشی قابل­توجهی روی هر دو گونه حشره آفت می­باشند که می‌توانند در برنامه‌های کنترل تلفیقی آفات به­کار گرفته شوند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of transgenic tobacco plant, Nicotiana tabacum expressing ribosome inactivating gene isolated from the bulbs of Dutch iris, Iris hollandica on biological parameters of Myzus nicotianae (Hem.: Aphididea) and Spodoptera exigua (Lep.: Noctuidae)

نویسنده [English]

  • Sh. Shahidi-Noghabi
Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.
چکیده [English]

The use of modified crop plants through gene transfer between different plant species plays an important role in pest control programs. We have investigated the insecticidal properties of two lines of tobacco transgenic plant consisting of IRIP (expressed with type 1 ribosome inactivating gene) and IRA (expressed with type 2 ribosome inactivating gene) against beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae) and tobacco aphid, Myzus nicotianane Blackman (Hemiptera: Aphididae). The transgenic tobacco plants expressing IRA increased mortality and decreased reproduction rate of tobacco aphid. The reproductive period of tobacco aphid fed on transgenic tobacco plant expressing IRA reduced in comparison with the control and IRIP. We did not observed any impact on reproduction parameters of the aphids treated with IRIP plant in comparison with control. Feeding second instar larvae of S. exigua on transgenic tobacco plant expressing IRA led to about 33.3% mortality. Weight of larvae and pupae as well as the rate of the emergence of adult S. exigua were significantly decreased in larvae fed on IRA in comparison with control. These results suggest that type-2 ribosome inactivating proteins serve as significant insecticidal factors on both insect pest and can be used in integrated pest control programs.

کلیدواژه‌ها [English]

  • Lepidoptera
  • Ribosome inactivating proteins
  • Hemiptera
  • Lectins

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