Characterization of digestive α- and β-glucosidases in the midgut of Plagiodera versicolora Laicharting (Coleoptera: Chrysomelidae)

نویسندگان

گروه گیاه‌پزشکی، دانشکده‌ی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.

چکیده

سوسک برگخوار بید Plagiodera versicolora یکی از آفات مهم درختان جنگلی در دنیا از جمله ایران می­باشد. در پژوهش حاضر، فعالیت آلفا و بتا- گلوکوزیداز در معده میانی حشره کاملP. versicolora  بررسی شد. نتایج نشان داد که فعالیت آلفا- گلوکوزیداز بیش­تر از بتا-گلوکوزیداز بود. pH بهینه برای فعالیت آلفا و بتا- گلوکوزیداز به­ترتیب 6 و 9 به­دست آمد. هم­چنین دمای بهینه فعالیت این دو آنزیم به­ترتیب 30 و 25 درجه سلسیوس محاسبه شد. غلظت 5/0 میلی‏مولار یون K+بیش­ترین تأثیر را در کاهش فعالیت آنزیم آلفا- گلوکوزیداز داشت و بهینه فعالیت آلفا- گلوکوزیداز در حضور یون Ca2+مشاهده شد. فعالیت آنزیم بتا- گلوکوزیداز با اضافه کردن یون‏های Mg2+وZn2+کاهش یافت. علاوه بر این، غلظت 5 میلی‏مولار یون K+ بیش­ترین تأثیر بازدارندگی را بر فعالیت بتا- گلوکوزیداز داشت و یون +Na (غلظت 5/0 میلی‏مولار) بیش­ترین تأثیر مثبت را بر فعالیت آنزیم داشت. مقدار Km آلفا و بتا گلوکوزیداز به­ترتیب 36/0 و 03/2 میلی‏مولار و مقدار Vmax به­ترتیب46/5 و 51/18 میکرومول بر دقیقه بر میلی‏گرم پروتئین به­دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Characterization of digestive α- and β-glucosidases in the midgut of Plagiodera versicolora Laicharting (Coleoptera: Chrysomelidae)

نویسندگان [English]

  • M. Shabarari
  • B. Naseri
Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
چکیده [English]

The imported willow leaf beetle, Plagiodera versicolora is one of the main pests of forest trees worldwide including Iran. Activities of α- and β-glucosidases in the midgut of P. versicolora adults were studied in this research. The results showed a higher α- glucosidase activity of adultsthan β-glucosidase. The optimal pHs for α- and β-glucosidases activities were found to be 6 and 9, respectively. Optimal temperatures for the enzymatic activities were recorded at 30 and 25 °C, respectively. Concentration of 0.5 mM K+ significantly minimized the activity of α- glucosidase and maximized the activity of α-glucosidase in presence of Ca2+. The activity of β-glucosidase was decreased by adding Mg2+ and Zn2+. 5 mM concentration of K+ had the most inhibitory impact on β-glucosidase activity, while 0.5 mM concentration of Na+ showed the most positive effect on the enzyme activity. The Km of midgut α- and β- glucosidase was 0.36 and 2.03 mM and the Vmax value was 5.46 and 18.51 μmol/min/mg protein, respectively.

کلیدواژه‌ها [English]

  • Plagiodera versicolora
  • α-glucosidase
  • β-glucosidase
  • Iran
  • Midgut

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