اثر عصاره متانولی Rosmarinus officinalis بر نوروپپتید فعال کننده قلب سخت پوستان، فعالیت آنزیم های گوارشی و شاخص های تغذیه ای کرم غوزه پنبه (Helicoverpa armigera (Lepidoptera: Noctuidae

نوع مقاله: مقاله کامل، فارسی

نویسندگان

گروه حشره شناسی کشاورزی، دانشکده کشاورزی دانشگاه تربیت مدرس، تهران، ایران

چکیده

اثر عصاره متانولی برگRosmarinus officinalis  روی لارو سن سوم کرم غوزه پنبه (Helicoverpa armigera (Hübner مورد مطالعه قرار گرفت. LC50 و LC20 آن به ترتیب برابر 12347و 5138 پی پی ام تخمین زده شد. 100 میکرولیتر از عصاره رزماری (با غلظتppm  5138) به 9/0 گرم غذای مصنوعی اضافه شد. پس از 72 ساعت، کارایی تبدیل غذای خورده ‌شده (ECI) ، کارایی تبدیل غذای هضم شده (ECD) ، نرخ مصرف نسبی (RCR) و نرخ رشد نسبی (RGR) کاهش یافت ولی میزان  شاخص تقریبی هضم شوندگی غذا (AD) اضافه شد. علاوه بر این میزان فعالیت آنزیم­ های آلفا آمیلاز، پروتئاز و لیپاز نیز در لارو سن سوم  H. armigera کاهش نشان داد. قرار دادن معده میانی در بافر محتوی نوروپپتید فعال کننده قلب سخت پوستان (CCAP) میزان فعالیت آنزیم لیپاز را افزایش داد. تغذیه از غذای مصنوعی حاوی عصاره رزماری میزان نوروپپتید فعال کننده قلب سخت پوستان را در مغز، همولنف و معده میانی کاهش داد. نتایج نشان داد، تغذیه از ماده غذایی حاوی عصاره رزماری، مانع آزاد سازی CCAP در معده میانی و مغز می­شود که این موضوع به نوبه خود منجر به کاهش میزان فعالیت آنزیم های آلفا آمیلاز، پروتئاز و لیپاز شد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Rosmarinus officinalis, leaf methanolic extract on crustacean cardioactive peptide, digestive enzyme activities and nutritional indices of Helicoverpa armigera (Lepidoptera: Noctuidae)

نویسندگان [English]

  • Vahid Mohammadi Gisour
  • Azam Mikani
  • Saeid Moharramipour
Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Efficacy of methanolic leaf extract of Rosmarinus officinalis on third instar larvae of cotton bollworm, Helicoverpa armigera (Hübner) was studied. The value of LC50 and LC20 were 5138 and 12347 ppm, respectively.  One hundred microliters of the Rosemary’s mehanolic extract at 5138 ppm was added to 0.9 gr artificial diet. Efficiency of digested food (ECD), efficiency of conversion of ingested food (ECI), relative consumption rate (RCR) and relative growth rate (RGR) were decreased after 72 h but approximate digestibility (AD) was increased. It also decreased α-amylase, protease and lipase activity in the midgut of third instar larvae of H. armigera. Incubation of dissected midgut with crustacean cardioactive peptide (CCAP) increased lipase activity. Feeding on artificial diet containing plant extract caused decreasing in CCAP level in the brain, hemolymph and midgut of the insect. The results showed feeding on diet containing rosemary’s extract inhibits release of CCAP in the midgut and brain that itself leads to reduction of α-amylase, lipase and protease activities.

کلیدواژه‌ها [English]

  • Helicoverpa armigera
  • Rosmarinus officinalis
  • crustacean cardioactive peptide
  • Digestive enzymes

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