بررسی اثر کنه‌کشی روغن و عصاره متانولی بذر منداب Eruca sativa روی (Tetranychus urticae (Red form)(Acari:Tetranichidae

نوع مقاله: مقاله کامل، فارسی

نویسندگان

1 دانشگاه تربیت مدرس، دانشکده کشاورزی، گروه حشره شناسی کشاورزی، تهران، ایران

2 دانشگاه تربیت مدرس، دانشکده کشاورزی، گروه علوم باغبانی، تهران، ایران

چکیده

در این پژوهش اثر کنه‌‌کشی روغن و عصاره‌ی متانولی بذر منداب Eruca sativa (Miller) (Brassicaceae) روی کنه Tetranychus urticae (Red form)(Acari:Tetranychidae)در شرایط آزمایشگاهی مورد ارزیابی قرار گرفت. تلفات کنه‌های بالغ بعد از 24 ساعت ارزیابی و مقادیر LC50 روغن و عصاره محاسبه شد. این مقادیر برای روغن و عصاره‌ی متانولی بذر منداب به ترتیب 3062 و 5674 میلی‌گرم بر لیتر به دست آمد. اندازه‌گیری میزان ایزوتیوسیانات روغن و عصاره متانولی بذر منداب با روش کروماتوگرافی مایع با کارآیی بالا (HPLC) نشان داد که میزان ایزوتیوسیانات اروسین در روغن منداب نسبت به عصاره متانولی آن بالاتر می‌باشد. همچنین آنالیز اسیدهای چرب روغن بذر منداب با استفاده از کروماتوگرافی گازی (GC) وجود 12 نوع اسید چرب را در روغن نشان داد که سطح اسید‌های چرب آراشیدونیک، اولئیک و گاما‌لینولنیک در آن بالا بود. با توجه به پایین بودن میزان ایزوتیوسیانات‌ اروسین و اسیدهای چرب در عصاره متانولی بذر منداب می­توان علت کنه‌‌کشی بیشتر روغن منداب را به نوع و مقدار ایزوتیوسیانات و اسیدهای چرب آن نسبت داد. بنابراین یافته‌های ما نشان داد که حضور فیتوکمیکال‌های فعال در روغن E. sativa نقش مهمی در اثر بخشی آن علیه کنه T. urticae  دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Acaricidal activity of Eruca sativa seed oil and its methanolic extract on Tetranychus urticae (Red form)(Acari:Tetranichidae)

نویسندگان [English]

  • Saeid Moharramipour 1
  • Maryam Masoumi 1
  • Mehdi Ayyari 2
1 Department of Agricultural Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2 Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
چکیده [English]

In this study, contact toxicity of Eruca sativa (Miller) (Brassicaceae) oil and its methanolic extract were examined on the adult female of Tetranychus urticae (Red form)) (Tetranychidae) at five concentrations and three replications set at 25 ± 1°C, 65 ± 5% RH and 16: 8 h (light: dark). The mortality was evaluated 24 h after treatment. The LC50s of E. sativa oil and the methanol extract were 3062 and 5674 mg L-1, respectively. Measurement of erucin as the main isothiocyanate compound in the oil and methanolic seed extract using high performance liquid chromatography (HPLC) showed that the amount of erucin in the oil was higher than methanol extract. Also, analysis of fatty acids in the oil using gas chromatography (GC) showed that there were 12 types of fatty acids in oil, high level of arachidonic, oleic and Ƴ-linolenic acids. Due to the biological activity and phytochemical analysis, it can be concluded that the higher amount of erucin and fatty acids in the oil, caused the higher acaricidal activity of E. sativa oil compared to methanolic extract. Therefore, our findings showed that the presence of main active phytochemicals in the E. sativa oil plays a decisive role in its efficacy.

کلیدواژه‌ها [English]

  • Eruca sativa oil
  • Isothiocyanate
  • Fatty acid
  • acaricid

Abbott, W. (1925) A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18(2), 265-267.

Aissani, N., Urgeghe, P. P., Oplos, C., Saba, M., Tocco, G., Petretto, G. L., Eloh, K., Menkissoglu-Spiroudi, U., Ntalli, N. & Caboni, P. (2015) Nematicidal activity of the volatilome of Eruca sativa on Meloidogyne incognita. Journal of Agricultural & Food Chemistry 63(27), 6120-6125.

Azaizeh, H., Kobaisy, M., Dakwar, S., Saad, B., Shaqir, I. & Said, O. (2007) Botanical pesticides as a source of safe bioacaricides for the control of Tetranychus cinnabarinus. Acta Phytopathologica et Entomologica Hungarica 42(1), 143-152.

Blažević, I. & Mastelić, J. (2008) Free and bound volatiles of rocket (Eruca sativa Mill.). Flavour & Fragrance Journal 23(4), 278-285.

Borek, V., Elberson, L. R., McCaffrey, J. P. & Morra, M. J. (1998)Toxicity of isothiocyanates produced by glucosinolates in brassicaceae species to black vine weevil eggs. Journal of Agricultural and Food Chemistry 46(12), 5318-5323.

Cardiet, G., Fuzeau, B., Barreau, C. & Fleurat-Lessard, F. (2012)Contact and fumigant toxicity of some essential oil constituents against a grain insect pest Sitophilus oryzae and two fungi, Aspergillus westerdijkiae and Fusarium graminearum. Journal of Pest Science 85(3), 351-358.

Chen, Y. & Dai, G. (2015)Acaricidal, repellent, and oviposition-deterrent activities of 2, 4-di-tert-butylphenol and ethyl oleate against the carmine spider mite Tetranychus cinnabarinusJournal of Pest Science 88(3), 645-655.

Choi, W. I., Lee, S. G., Park, H. M. & Ahn, Y. J. (2004)Toxicity of plant essential oils to Tetranychus urticae (Acari: Tetranychidae) and Phytoseiulus persimilis (Acari: Phytoseiidae). Journal of Economic Entomology 97(2), 553-558.

De Oliveira, J. L., Campos, E. V. R., Bakshi, M., Abhilash, P. C. and Fraceto, L. F. (2014)Application of nanotechnology for the encapsulation of botanical insecticides for sustainable agriculture: prospects and promises. Biotechnology Advances 32(8), 1550-1561.

Demirel, N., Kurt, S., Gunes, U., Uluc, F. & Cabuk, F. (2009)Toxicological responses of confused flour beetle, Tribolium confusum du Val (Coleoptera: Tenebrinoidea) to various isothiocyanate compounds. Asian Journal of Chemistry 21(8), 6411-6416.

Dubey, N. K., Shukla, R., Kumar, A., Singh, P. & Prakash, B. (2010)Prospects of botanical pesticides in sustainable agriculture. Current Science 98(4), 479-480.

Finney, D. J. (1971) Probit Analysis, 3rd ed. 333 pp. Cambridge University Press, London.

Garg, G. & Sharma, V. (2014) Eruca sativa (L.):Botanical description, crop improvement, and medicinal properties. Journal of Herbs, Spices & Medicinal plants 20(2), 171-182.

Isman, M. B. (2006)Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51, 45-66.

Khater, H. F. (2012)Prospects of botanical biopesticides in insect pest management. Pharmacologia 3(12), 641-656.

Kim, S. J., Jin, S. & Ishii, G. (2004) Isolation and structural elucidation of 4-(β-D-glucopyranosyldisulfanyl) butyl glucosinolate from leaves of rocket salad (Eruca sativa L.) and its antioxidative activity. Bioscience, biotechnology, and biochemistry 68(12), 2444-2450.

Mahdavian, A. (2016)Insecticidal activity of some botanical extracts against pistachio psyllid Agonoscena pistaciae (Hem: Psyllid) in field and laboratory conditions. MS.C thesis, Department of Entomology, Tarbiat Modares University, Tehran, Iran. 89 p.

Mansour, E. E., Mi, F., Zhang, G., Jiugao, X., Wang, Y. & Kargbo, A. (2012)Effect of allylisothiocyanate on Sitophilus oryzae, Tribolium confusum and Plodia interpunctella: Toxicity and effect on insect mitochondria. Crop Protection 33, 40-51.

Mattner, S., Porter, I., Gounder, R., Shanks, A., Wren, D. & Allen, D. (2008)Factors that impact on the ability of biofumigants to suppress fungal pathogens and weeds of strawberry. Crop Protection 27(8), 1165-1173.

Matusheski, N. V., Swarup, R., Juvik, J. A., Mithen, R., Bennett, M. , Jeffery, & E. H. (2006)Epithiospecifier protein from broccoli (Brassica oleracea L. Ssp. Italica) inhibits formation of the anticancer agent sulforaphane. Journal of Agricultural & Food Chemistry 54(6), 2069-2076.

Metcalf, l. C., Schmitz, A. A. & Pelka, J. R. (1966)Rapid preparation of methyl esters from lipid for gas chromatography. Analytical Chemistry 38(3), 514-515.

Morra, M. & Kirkegaard, J. (2002)Isothiocyanate release from soil-incorporated brassica tissues. Soil Biology & Biochemistry 34(11), 1683-1690.

Nicolopoulou-Stamati, P., Maipas, S., Kotampasi, C., Stamatis, P. & Hens, L. (2016)Chemical pesticides and human health: the urgent need for a new concept in agriculture. Frontiers in Public Health, 4, 148.

ObengOfori, D. (1995)Plant oils as grain protectants against infestations of Cryptolestes pusillus and Rhyzopertha dominica in stored grain. Entomologia Experimentalis et Applicata 77(2), 133-139.

Paes, J., LRD'A, F., Dhingra, O., Cecon, P. & Silva, T. (2012)Insecticidal fumigant action of mustard essential oil against Sitophilus zeamais in maize grains. Crop Protection 34, 56-58.

Pavela, R. & Benelli, G. (2016)Essential oils as ecofriendly biopesticides? Challenges and constraints. Trends in Plant Science 21(12), 1000-1007.

Pavela, R. (2015)Essential oils for the development of eco-friendly mosquito larvicides: a review. Industrial Crops & Products 76, 174-187.

Rattan, R. S. (2010) Mechanism of action of insecticidal secondary metabolites of plant origin. Crop Protection 29(9), 913-920.

Rosa, E. & Rodrigues, P. (1999)Towards a more sustainable agriculture system: The effect of glucosinolates on the control of soil-borne diseases. The Journal of Horticultural Science & Biotechnology 74(6), 667-674.

Sabelis, M. W. & Dicke, M. (1985)Long-range dispersal and searching behaviour. pp. 141-160 In: Helle, W. & Sabelis, M. W. (Eds.) Spider Mites: Their Biology, Natural Enemies and Control. Elsevier Amsterdam.

Santos, E. A. d., Carvalho, C. M. d., Costa, A. L., Conceição, A. S., Moura, F. d. B. P. & Santana, A. E. G. (2012)Bioactivity evaluation of plant extracts used in indigenous medicine against the snail, Biomphalaria glabrata, and the larvae of Aedes aegypti. Evidence-Based Complementary & Alternative Medicine.

Seifi, R., Moharramipour, S. & Ayyari, M. (2018)Acaricidal activity of different fractions of Moringa peregrina on two spotted spider mite Tetranychus urticae (Acari: Tetranychidae). Industrial Crops & Products 125, 616-621.

Shi, C. H., Hu, J. R., Xie, W., Yang, Y. T., Wang, S. L. & Zhang, Y. J. (2017)Control of Bradysia odoriphaga (Diptera: Sciaridae) with allyl isothiocyanate under field and greenhouse conditions. Journal of Economic Entomology 110(3), 1127-1132.

Wathelet, J. P., Iori, R., Leoni, O., Rollin, P., Quinsac, A. & Palmieri, S. (2004)Guidelines for glucosinolate analysis in green tissues used for biofumigation. Agroindustria 3(3), 257-266.

Wink, M. (2010)Annual Plant Reviews, Functions and Biotechnology of Plant Secondary Metabolites (Vol. 39). John Wiley & Sons.

Wu, H., Zhang, G. A., Zeng, S. & Lin, K. C. (2009)Extraction of allyl isothiocyanate from horseradish (Armoracia rusticana) and its fumigant insecticidal activity on four stored‐product pests of paddy. Pest Management Science 65(9), 1003-1008.

Zhang, W.G. (2016) Aqueous extraction and nutraceuticals content of oil using industrial enzymes from microwave puffing-pretreated Camellia oleifera seed powder. Food Science and Technology Research 22(1), 31-38.