بررسی خواص حشره کـشی نانو امولسیون اسانس پونه، (Mentha longifolia L. (Lamiaceae علیه سوسـک چهار نقطه ای حبوبات، (Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae

نوع مقاله: مقاله کامل، فارسی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

2 موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران.

چکیده

امروزه نانو امولسیون­ها به دلیل خواص فیزیکی و شیمیایی منحصر به فردی مانند سایز کوچک ذرات، ثبات فیزیکی طولانی مدت و شفافیت مورد توجه بسیاری از محققین قرار گرفته ­اند. در این تحقیق سمیت تنفسی اسانس پونه Mentha longifolia و نانوامولسیــون آن علیه سوسک چهارنقطــه ­ای حبــوبات Callosobruchus maculatus مطالــعه شد. آزمایش­ها در شرایط آزمایشگاهی، دمای 2±27 درجه سلسیوس، رطوبت نسبی 5±65 و در تاریکی انجام شد. اسانس پونه در ساعات اولیه پس از انجام آزمایش سمیت تنفسی بالاتری نسبت به نانوامولسیون داشت. در مرحله حشره کامل مقدار LC50 اسانس و نانوامولسیون به ترتیب برابر با 1/10 و 1/16 میکرولیتر بر لیتر هوا برآورد شد. مقدارLC50  در مرحله جنینی برای اسانس برابر با 9/4 میکرولیتر بر لیتر هوا و برای نانو امولسیون 4/9 میکرولیتر بر لیتر هوا تعیین شد. در غلظت 25 میکرولیتر بر لیتر هوا، میزان LT50 اسانس و نانوامولسیون به ترتیب برابر با 2/2 و 1/11 روز بود. در غلظت 1/10 میکرولیتر بر لیتر هوا اسانس معمولی دوامی نداشت، ولی نانوامولسیون دوام خوبی (روز 04/9 =LT50) داشت. در غلظت 9 میکرولیتر بر لیتر هوا مدت زمانی که طول کشید 50 درصد تخم­ها در اثر اسانس معمولی دچار مرگ ­و ­میر شوند، 7/3 روز برآورد شد. میزان LT50 نانوامولسیون در این غلظت 09/17 روز بود. در غلظت 9/4 میکرولیتر بر لیتر هوا میزان LT50 نانوامولسیون 8/12 روز بود، در حالیکه اسانس معمولی فاقد دوام بود. میانگین قطر ذرات نانوامولسیون با استفاده از میکروسکوپ الکترونی (TEM) حدود 36-10 نانومتر تعیین شد. بر اساس نتایج به دست آمده، بکارگیری فرم نانوامولسیون اسانس گیاه پونه می­تواند باعث افزایش سمیت تنفسی و دوام آن شود.

کلیدواژه‌ها


عنوان مقاله [English]

Study on insecticidal properties of nanoemulsion Mentha longifolia L. (Lamiaceae) essential oil against Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae)

نویسندگان [English]

  • Mojdeh Louni 1
  • Jahanshir Shakarami 1
  • Maryam Negahban 2
1 Department of Plant protection, Faculty of Agriculture Science, University of Lorestan, Khorramabad, Iran
2 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organi-zation (AREEO), Tehran, Iran
چکیده [English]

Nowadays, nanoemulsions are considered by many researchers because of their unique physiochemical properties such as small particle size, long-term physical stability and transparency. In this research, fumigant toxicity of Mentha longifolia L. essential oil and its nanoemulsion against Callosobruchus maculatus (Fabricius) has been studied. The experiments were conducted at laboratory conditions at 27±2°C temperature, 65±5% relative humidity and darkness. Essential oil of M. longifolia had higher fumigant toxicity than nanoemulsion, during early hours after treatment. The LC50 values of oil and nanoemulsion for the adults of the pest were 10.1 and 16.1 µl/l, respectively. The values of LC50 were 4.9 and 9.4 µl/l for oil and nanoemulsion during incubation period, respectively. In concentration of 25 µl/l, LT50 of M. longifolia essential oil and nanoemulsion were 2.2 and 11.1 days, respectively. In concentration of 10.1 µl/l, essential oil did not have durability, but this parameter of nanoemulsion is good (LT50= 9.04 days). At 9 µl/l of essential oil, the average time for the mortality of 50% eggs was 4.7 days. The LT50 of nanoemulsion at this concentration was 17.09 days. At 4.9 µl/l of nanoemulsion, the LT50 was 12.8 days, while essential oil did not have durability. The average diameter of nanoemulsion particles has been estimated about 10-36 nm by using transmission electron microscope (TEM). By using laser light scattering (SEMATech), this value was 234 nm. Considering obtained results, using nanoemulsion form of M. longifolia essential oil increase its fumigant toxicity and durability.

کلیدواژه‌ها [English]

  • Callosobruchus maculatus
  • essential oil
  • Insecticidal
  • Mentha longifolia
  • Nanoemulsion

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