بررسی مقاومت ارقام مختلف هلو نسبت به کنه تارتن دولکه‌ای Acari: Tetranychidae) Tetranychus urticae Koch) در شرایط آزمایشگاهی

نوع مقاله: مقاله کامل، فارسی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهرکرد، ایران

2 Department of Plant Protection, Shahrekord University

3 Shahid Chamran University

4 Department of Plant Protection, Agricultural and Natural Resources Research Center, Chaharmahal and Bakhtiari, Iran

چکیده

کنه تارتن دولکه‌­ای Tetranychus urticae Koch یکی از آفات مهم هلو در ایران می‌­باشد. این مطالعه به منظور ارزیابی مقاومت پنج رقم هلو (Redtap، G. H. Hale، Kardi، Elberta و Springtimes) نسبت به این آفت انجام شد. مقاومت این ارقام در شرایط آزمایشگاهی در دمای 1±27 درجه سلسیوس، رطوبت نسبی10±50 درصد و دوره نوری D12: L12 ساعت، به روش دیسک برگی (رهاسازی پنج کنه ماده بالغ بارور روی دیسک­‌هایی از برگ ارقام هلو و شمارش تعداد تخم کنه، مرگ و میر و ارزیابی میزان خسارت آن) مورد بررسی قرار گرفت. همچنین تراکم کرک‌­ها در سطح رویی و زیرین برگ، غلظت ترکیبات فنلی و میزان عناصر غذایی موجود در برگ ارقام مختلف مورد محاسبه قرار گرفت. بر اساس نتایج بدست آمده، بیشترین میزان خسارت وارده به دیسک برگی در ارقام جی ­اچ ­هیل و کاردی و کمترین میزان آن در ارقام البرتا، ردتاپ و اسپرینگ­تایمز مشاهده شد. همچنین نتایج نشان داد، تراکم کرک‌­ها در سطح رویی و زیرین برگ­ها بین ارقام مختلف هلو اختلاف معنی­‌داری نداشت. درنتیجه این پراسنجه نقشی در ایجاد مقاومت نسبت به این کنه ندارد. به علاوه، رابطه­ای بین غلظت ترکیبات فنلی و مقاومت نسبت به کنه تارتن دولکه­ای وجود نداشت. به طور کلی نتایج نشان داد که ارقام ردتاپ، البرتا و اسپرینگ ­تایمز مقاوم و ارقام جی ­اچ ­هیل و کاردی حساس­ترین میزبان­‌ها نسبت به کنه تارتن دولکه­ای در میان ارقام مورد بررسی بودند و به نظر می­رسد عدم تعادل عناصر غذایی به ویژه عنصر پتاسیم عامل اصلی مقاومت به این آفت است.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation on resistance of different peach cultivars to two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) in laboratory conditions

نویسندگان [English]

  • Alireza Nemati 1
  • Elham Riahi 2
  • Parviz Shishehbor 3
  • Zarir Saeidi 4
1 Plant Protection Dept., Agricultural Coll., Shahrekord Univ., Shahrekord, Iran
2 Shahrekord University
3 Shahid Chamran University
4 Department of Plant Protection, Agricultural and Natural Resources Research Center
چکیده [English]

Two-spotted spider mite (TSSM), Tetranychus urticae Koch is an important pest of peach in Iran. This study was conducted to estimate the resistance of five peach cultivars (Redtap, G.H.Hale, Kardi, Elberta and Springtimes). Resistance of different cultivars to this pest was evaluated at 27 ± 1°C, 50 ± 10 %RH and 12 L: 12 D h., using leaf disk bioassay with introducing five adult female mites on leaf disk and counting number of eggs, mite mortality and host damage. Moreover, trichomes density on upper and underside of the leaves, concentration of phenolic compounds and percentage of leaf nutrients were measured. The results showed that the highest plant damage was on G.H.Hale and Kardi cultivars, and the lowest on Elberta, Redtap and Springtimes. The findings also indicated that the density of trichomes on upper and underside of the leaves was not significantly different among cultivars. Therefore, this parameter has no role in resistance to TSSM. Furthermore, there was no relationship between concentration of phenolic compounds and resistance to TSSM. Altogether results revealed that Redtap, Elberta and Springtimes were the most tolerant, whereas G.H.Hale and Kardi were the most susceptible cultivars and nutrient imbalance especially potassium is probably main factor of the resistance against TSSM.  

کلیدواژه‌ها [English]

  • nutrient elements
  • Phenolic compounds
  • trichome
  • Leaf disk

Ania, O. J., Rodriguez, G. & Knavel, D. E. (1972) Characterizing resistance to Tetranychus urticae in tomato. Journal of Entomologist 65, 641–643.

Anonymous. (2019) Food and Agriculture Organization. Available from: http://www.fao.org/faostat/en/#data/QC(accessed in: 1 April 2019).

Banerjee, M. K. & Kalloo, K. (1988) Role of phenols in resistance to tomato leaf curl virus, Fusarium wilt and fruit borer in Lycopersicon. Current Science 58(1), 575–578.

Barnes, R. B., Richardson, D., Berry, J. W. & Hood, R. L. (1945) Flame photometry a rapid analytical procedure. Industrial and Engineering Chemistry, Analytical Edition 17(10), 605–611.

Bolland H. R., Gutierrez J. & Flechtmann C. H. W. (1998) World catalogue of the spider mite family (Acari: Tetranychidae). 392 pp. Leiden: Brill Academic Publishers.

Cortesero, A. M., Stapel, J. O. & Lewis, W. J. (2000) Understanding and manipulating plant attributes to enhance biological control. Biological Control 17, 35–49.

Egas, M. & Sabelis M. W. (2003) Adaptive learning in arthropods: spider mites learn to distinguish food quality. Experimental & Applied Acarology 30 (3), 233-247.

Elser J. J., Fagan W. F., Denno R. F.,  Dobberfuhl, D. R., Folarin, A., Huberty, A., Interlansi, S., Kilham, S. S., McCauley, E., Schulz, K. L., Siemann E. H. & Sterner, R. W. (2000) Nutritional constraints in terrestrial and freshwater food webs. Nature 408, 578–580.

Fritzsche, R., Wolffgang, H., Reiss, E. & Thiele, S. (1980) Untersuchungen zu den Ursachen sortenbedingter BefaUsunterschiede von Apfelbaiumen mit Oligonychus ulmi Koch. Archiv fur Phytopathologie und Pflanzenschutz 16, 193–198.

Gimenes-ferre, R. M., Sheerens J. C. & Erb, W. A. (1993) In vitro screening of 76 strawberry cultivars for two- spotted spider mite. Horticultural Science 28, 841–844.

Hare, J. D. (2011) Ecological role of volatiles produced by plants in response to damage by herbivorous insects. Annual Review of Entomology 56, 161–80.

Heinz, K. M. & Zalom, F. G. (1995) Variation in trichome based resistance to Bemisia argantifolii (Homoptera: Aleyrodidae) oviposition on tomato. Journal of Economic Entomology 88(5), 1494–1502.

Huberty A. F. & Denno R. F. (2006) Consequences of nitrogen and phosphorus limitation for the performance of two planthoppers with divergent life-history strategies. Oecologia149, 444–455.

Jackson, M. L. (1962) Soil chemical analysis. 498 pp. Englewood Cliffs, NJ, USA, Prentice- Hall, INC.

Johnson, H. B. (1975) Plant pubescence: an ecological perspective. The Botanic Review 41, 233–258.

Kielkiewicz, M. (1994) The appearance of phenolics in tomato leaf tissues exposed to spider mite attack. Acta Horticultural 381, 637–690.

Kumar, H. (1992) Inhibition of oviposition responses of Chilo partellus (Lepidoptera: Pyralidae) by the trichomes on the lower surfaces of maize cultivar. Journal of Economic Entomology 85(5), 1736–1739.

Lattanzio, V., Terzano, R., Cicco, N., Cardinali, A., Di Venere, D. & Linsalata,V. (2005) Seed coat tannins and bruchid resistance in stored cowpea seeds. Journal of the Science of Food and Agriculture 85 (5), 839–846.

Leeuwen, T., Vontas, J., Tsagkarakou, A. & Tirry, L. (2009) Mechanisms of acaricide resistance in the two-spotted spider mite Tetranychus urticae. Systematic and Applied Acarology 13, 347–393.

Levin, D. A. (1973) The role of trichomes in plant defense. Quarterly Review of Biology 48, 3–15.

Luczynski, A., Isman, M. B., Raworth, D. A. & Chan, C. K. (1990) Chemical and morphological factors of resistance against the two- spotted spider mite in bean strawberry. Journal of Economic Entomology 83, 564–569.

Malakooti, M. J. (1998). Critical limit of elements for strategic products. 87 pp. Agricultural Education Publication. [In Persian]

McAuslane, H. J., Johnson, F. A., Colvin, D. L. & Sojack, B. (1995) Influence of foliar pubescence on abundance and parasitism of Bemisia argentifolii (Homoptera: Aleyrodidae) on soybean and peanut. Environmental Entomology 24, 1135-1143.

Mohammadi, S. (2008) Investigation on the resistance of different white and red bean genotypes to two-spotted spider mites, Tetranychus urticae Koch in Shahrekord. MSc Thesis, Shahid Chamran University, 96 pp.

Motahari, M., Kheradmand, K., Roustaee, A.M. & Talebi. A.A. (2014) The impact of cucumber nitrogen nutrition on life history traits of Tetranychus urticae (Koch) (Acari: Tetranychidae). Acarologia 54 (4), 443-452.

Murphy, J. & Riley, J. P. (1962) A modified single solution for determination of phosphate in natural waters. Analytica Chimica Acta 27, 35–36.

Noorbakhsh, S. H. (2012) Investigation on almond mites fauna and population dynamics of tetranychid mites in Shahrekord city. 31pp. Final report of the research project of Agricultural Research Center of Chaharmahal va Bakhtiyari province. [In Persian]

Oliveira S.R., Veto A.G., Nóbrega, J. A. & Jones, B. T. (2010) Determination of macro- and micronutrients in plant leaves by high-resolution continuum source flame atomic absorption spectrometry combining instrumental and sample preparation strategies. Spectrochimica Acta Part B: Atomic Spectroscopy 65 (4), 316–320.

Parvez M.M., Tomita-Yokotani K., Fujii Y., Konishi T. & Iwashina T. (2004) Effects of quercetin and its seven derivatives on the growth of Arabidopsis thaliana and Neurospora crassa. Biochemical Systematic and Ecology 32, 631–635.

Rakha, M., Bouba, N., Ramasamy S., Regnard, J. L. & Hanson, P. (2017) Evaluation of wild tomato accessions (Solanum spp.) for resistance to two-spotted spider mite (Tetranychus urticae Koch) based on trichome type and acylsugar content. Genetic Resources and Crop Evolution 64 (5), 1011–1022.

Riahi, E., Nemati, A., Shishehbor, P. & Saeidi, Z. (2011) Population growth parameters of the two-spotted spider mite, Tetranychus urticae, on three peach varieties in Iran. Acarologia 51(4), 473–480.

Riahi, E., Shishehbor, P., Nemati, A. & Saeidi, Z. (2013) Temperature Effects on Development and Life Table Parameters of Tetranychus urticae (Acari: Tetranychidae). Journal of Agricultural Science and Technology 15, 661- 672.

Sadasivam, S. & Manickam, A. (1996) Biochemical methods. 2th ed. 256 pp. New Age International Publisher.

Saeidi Z. (2011b) Study on Resistance of almond cultivars to spider mites. Iran: Chaharmahal & Bakhtiari Agricultural and Natural Resources Research Center. No. 86004. pp. 15.

Saeidi, Z. (2006) Nature of resistance to two- spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) in Lycopersicon species. Ph. D. Thesis, University of Agricultural Sciences, Bangalore, India. 159pp.

Saeidi, Z. (2011a) Reaction of different almond cultivars to Schizotetranychus smirnovi in Saman region, Chaharmahal va Bakhtiyari provine. Plant Protection 3(2), 111–122.

Saeidi, Z. (2014) Investigation on resistance of different almond cultivars/ genotypes to two-spotted spider mite, Tetranychus urticae Koch in laboratory and greenhouse condition. Journal of Entomological Research 5(20), 353–363. 

SAS Institute (2001) SAS/STAT: Users guide. 502 p. SAS Institute, Cary, North Carolina, USA.  

Schoonhoven, L. M., van Loon, J. J. A. & Dicke, M. (2005) Plant structure: the solidity of anti-herbivore protection. pp. 29–47 in Louis, M., Schoonhoven, L. M., van Loon, J. J. A. & Dicke, M.(Eds) Insect-plant Biology. 456 pp. Oxford University Press.

Sharma, B. L. & Pande, Y. D. (1986) A study of relationship between the population of Tetranychus neocaledonicus Andre (Acarina: Tetranychidae) and external characteristics of cucurbit leaves and their NPK contents. Journal of Advanced Zoology 7, 42–45.

Sharma, H. C., Sujana, G. & Rao, D. M. (2009) Morphological and chemical components of resistance to pod borer, Helicoverpa armigera in wild relatives of pigeonpea. Arthropod-Plant Interact 3, 151–61;

Snyder, J. C. & Carter, C. D. (1984) Leaf trichomes and resistance of Lycopersicon hirsutum and Lycopersicon esculentum to spider mite. Journal of American Society of Horticultural Sciences 109 (6), 837–843.

Southwood, R. (1986) Plant surfaces and insects- an overview. pp 1–22 in Juniper, B. & Southwood, R. (Eds) Insects and the Plant Surface. Edward Arnold, Oxford.

SPSS (2009) SPSS 17 for Windows, Users Guide. SPSS Inc., Chicago.

Tulisalo, U. (1971) Free and bound amino acids of three host plant species and various fertilizer treatments affecting the fecundity of the two-spotted spider mite, Tetranychus urticae Koch (Acarina, Tetranychidae ). Annals Entomologici Fennici 37, 155–163.

Urabe, J., Naeem, S., Raubenheimer, D. & Elser, J. J. (2010) The evolution of biological stoichiometry under global change. Oikos 119, 737–740.

Van- Impe, G. & Hance, T. (1993) A technique for testing varietal susceptibility to the mite Tetranychus urticae: applied to bean, cucumber, tomato and strawberry. Agronomic 13(8), 739– 749.

War, A. R., Paulraj, M. G., Ahmad, T., Buhroo, A. A., Hussain B., Ignacimuthu, S. & Sharma, H. C. (2012) Mechanisms of plant defense against insect herbivores. Plant Signaling & Behavior 7 (10), 1306–1320.

Weston, P. A., Johnson, D. A., Burton, H. T. & Snyder, J. C. (1989) Trichome secretion composition, trichome densities and spider mite resistance of ten accession of Lycopersicon hirsutum. Journal of American Society of Horticultural Sciences 114, 492–498.

Wilde, G., Thomas, W. & Hall, H. (1991) Plant resistance to two-spotted spider mite (Acari: Tetranychidae) in raspberry cultivars. Journal of Economic Entomology 84, 251–255.

Wilson, L. J. (1994) Plant- quality effect on life history parameters of the two spotted spider mite (Acari: Tetanychidae) on cotton.  Journal of Economic Entomology 87(6), 1556–1673.

Yousefi, M. & Dorri, H. (2006) Evaluation of resistance and its related mechanisms to two-spotted spider mite in 36 genotypes of green beans in greenhouse condition. 17th Iranian Plant Protection Congress, p. 215.

Zehnder C. B. & Hunter M. D. (2009) More is not necessarily better: the impact of limiting and excessive nutrients on herbivore population growth rates. Ecological Entomology34, 535–543.