Non-symbiotic association of Citrobacter freundii and Staphylococcus succinus with the entomopathogenic nematode Steinernema feltiae

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان

2 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه مراغه

چکیده

نماتودهای خاکزی متعلق به خانواده Steinernematidae (Nematoda: Rhabditida) پارازیت های اجباری و کشنده حشرات بوده که معمولا تحت عنوان نماتودهای بیمارگر حشرات (Entomopathogenic nematodes) EPNs، از آن­ها نام برده می شود.  این گروه از نماتودها به طور طبیعی با باکتری های جنس Xenorhabdus رابطه همزیستی داشته و نماتود مرحله سوم آلوده کننده یا Infective Juvenile، این باکتری ها را در یک وزیکول اختصاصی در روده­ی خود حمل می کنند. در این مطالعه وجود دو گونه از باکتری های غیر همزیست از لاشه حشرات آلوده به نماتود بیمارگر حشرات Steinernema feltiae گزارش می گردد. شناسایی باکتری های مذکور بر اساس آزمون های بیوشیمیایی و تجزیه و تحلیل نسب شناختی ترادف ژن rRNA-s16 انجام گرفت و در نهایت دو گونه باکتری به عنوان Citrobacter freundiiو Staphylococcus succinus شناسایی شدند. مطالعه اخیر تاییدی بیشتر بر وجود ارتباط بین باکتری های غیر همزیست با نماتودهای بیمارگر حشرات و توانایی نماتودها در انتقال آن­ها می باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Non-symbiotic association of Citrobacter freundii and Staphylococcus succinus with the entomopathogenic nematode Steinernema feltiae

نویسندگان [English]

  • Naser Eivazian Kary 1
  • Zhila Alizadeh 2
2 Department of Plant Protection, Faculty of Agriculture, Maragheh University
چکیده [English]

Soil-dwelling nematodes from Steinernematidae family are obligate parasites of insects and usually referred to as entomopathogenic nematodes (EPNs). These nematodes are symbiotically associated with entomopathogenic bacteria Xenorhabdus spp. The bacterial symbionts are carried monoxenically in a special vesicle in the infective juveniles (IJs). In the present study we report the isolation of two species of non-symbiotic bacteria from infected insect cadavers by the EPN, Steinernema feltiae. Galleria mellonella L. larvae were exposed to surface sterilized infective juveniles of S. feltiae and transferred to sterile Petri dishes for a further 24 hours. Hemolymph was collected and streaked onto both MacConkey and NBTA agar. Bacteria were identified using biochemical and phylogenetic analysis. 16S-rRNA gene sequence based maximum parsimony, maximum likelihood and neighbour joining phylogenetic analyses were conducted. Two non-symbiotic species including Citrobacter freundii and Staphylococcus succinus were identified and reported to be associated with S. feltiae. Our results provide further evidence for the existence of non-symbiotic bacteria associated with EPNs infection. 

کلیدواژه‌ها [English]

  • Entomopathogenic nematode
  • Steinernema feltiae
  • Citrobacter freundii
  • Staphylococcus succinus

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