واکنش پروتئوم لوبیا در اثر تغذیه کنه تارتن دولکه‌ای Tetranychus urticae Koch با رویکرد تکنیک پروتئومیکس

نوع مقاله: مقاله کامل، فارسی

نویسنده

بخش علوم کشاورزی، دانشگاه پیام‌نور

چکیده

کنه تارتن دولکه­ای،Koch  Tetranychus urticae، از مهمترین آفات لوبیا محسوب می­گردد که باعث خسارت وسیعی روی این گیاه می‌شود. مطالعه حاضر به منظور بررسی پاسخ پروتئینی گیاه لوبیا در حضور و عدم حضور آفت کنه تارتن دولکه­ای با استفاده از روش الکتروفورز دوبعدی صورت گرفت. جهت تعیین مقدار کمی پروتئین­ها از روش برادفورد استفاده گردید. نتایج نشان داد که در اثر تنش تغذیه­ای آفت، مقدار کمی پروتئین کل نسبت به حالت شاهد کاهش می­یابد. 266 لکه پروتئینی با رنگ­آمیزی کوماسی‌بلو R-250، از نمونه برگی لوبیا شناسایی گردید. الکتروفورز دوبعدی پروتئین تحت تنش تغذیه­ای آفت نشان­دهنده اختلافاتی در شدت بیان لکه­های پروتئینی برگ نسبت به گیاه در شرایط شاهد بود. به‌طورکلی تعداد هشت لکه­ پروتئینی نسبت به شاهد دارای کاهش بیان و تعداد چهار لکه بیان بیشتری نشان دادند. اکثر پروتئین­های شناخته شده دارای ارتباط مستقیم با سیستم دفاعی در برابر تنش بودند و نقش مهمی در این زمینه داشتند. از جمله این پروتئین­ها HSP، مالات دهیدروژناز و پلی‌فنل‌اکسیداز بودند. این نتایج شواهدی مبنی بر پاسخ سلول‌های مواجه شده با تنش برای ایجاد مقاومت به تغذیه آفت است. نتایج این مطالعه پس از بررسی الگوی باندی به کمک الکتروفورز یک بعدی (SDS-PAGE) نیز تنوع در بیان باندها در شرایط شاهد و شرایط تحت تیمار با آفت کنه تارتن دولکه‌ای را نشان داد. 

کلیدواژه‌ها


عنوان مقاله [English]

Response of Common Bean proteome to Two-Spotted Spider Mite Tetranychus urticae Koch using Proteomics Techniques

نویسنده [English]

  • Mehdi Kakaei
Department of Agriculture, Payame Noor University
چکیده [English]

Two-spotted spider mite, Tetranychus urticae Koch, is one of the most important pests of Common bean, which causes severe damage on the plants. The purpose of the current study, was determining the bean proteome response to presence and nonpresence of T. urticae using Two-dimensional gel electrophoresis. Bradford method was used to determine the amount of proteins. The results showed that under a nutritional stress, total amount of protein was slightly reduced in comparison with the control. 266 protein spots were identified from bean leaf samples painted by Coomassie Brilliant BlueR-250. Two-dimensional electrophoresis of proteins under a pest nutritional stress showed differences in expression level of leaf protein spots on the control. Overally, eight protein spots lower than the control, had decrease of expression and high expression was showed by four spots. Most of the
recognized proteins were related to a direct relationship with system defenses against the stress
including HSP, malate dehydrogenase and polyphenol oxidase. These results confirms the response of cells exposed to stress for resistance to the pest feeding. The bands pattern with SDS-PAGE, showed the variation in the expression of bands, in control conditions and the conditions under the treatment with Two-spotted spider mite.

کلیدواژه‌ها [English]

  • Two-dimensional gel electrophoresis
  • Leaf protein
  • Phaseolus vulgaris
  • Protein expression
  • Two-spotted spider mite

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