مطالعه مولکولی پسیل‌های زبان‌گنجشک، (Psyllopsis (Hemiptera: Liviidae و باکتری Wolbachia همزیست داخلی آن‌ها

نوع مقاله : مقاله کامل، انگلیسی

نویسندگان

1 گروه تنوع زیستی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران.

2 گروه بیوتکنولوژی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران.

چکیده

سه گونه پسیل زبان­ گنجشک، Psyllopsis repens Loginova 1963، Psyllopsis securicola Loginova 1963  و  Psyllopsis machinosus Loginova 1963 که در آسیای مرکزی و اروپا گسترش دارند، از آفات مهم زبان­ گنجشک در مناطق شهری ایران محسوب می­شوند. حشرات بالغ و پوره ­ها، و همچنین میزبان و زیست ­شناسی آن­ها بسیار شبیه به هم است. به عبارتی دیگر، شناسایی ریخت­ شناختی آن­ها، به ویژه ماده ­ها و حشرات بالغ آسیب­ دیده که از روی تله­ های چسبنده جمع­ آوری می­شوند، دشوار است. بنابراین، یک روش شناسایی سریع و دقیق مورد نیاز است. در این مقاله توالی دو ژن سیتوکروم c اکسیداز زیر واحد یک (mtCOI) و سیتوکروم (b (cytb به عنوان نواحی بارکد و یک ژن wsp مربوط به ژنوم باکتری Wolbachiaهمزیست داخلی آن­ها (ولباکیا) در سه گونه مورد مطالعه بررسی شد. نتایج مقایسه­ های ژنتیکی نشان داد که ژن­ های cytb و mtCOI، به ترتیب، بیشترین تنوع بین­ گونه­ای را نشان می­ دهند. بر اساس نتایج، دو گونة P. machinosus و P. repens که روی یک میزبان با هم زندگی می­ کنند، احتمالاً به ­وسیله یک استرین باکتری ولباکیا آلوده شده ­اند.

کلیدواژه‌ها

عنوان مقاله [English]

Molecular study of ash psyllids, Psyllopsis (Hemiptera: Liviidae), and their Wolbachia endosymbiont

نویسندگان [English]

  • M. R. Lashkari 1
  • S. Mirzaei 2
  • Roghayeh Shamsi Gushki 1
1 Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
2 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
چکیده [English]

Three ash psyllid species, Psyllopsis repens Loginova 1963, Psyllopsis securicola Loginova 1963 and Psyllopsis machinosus Loginova 1963, which have been distributed in the Central Asia and Europe, are important pests on genus Fraxinus spp. in the urban area of Iran. They have similar adult and larval morphology as well as same host and biology. On the other hand morphologically identification of adults and nymph, especially for females and damaged adults by removal from sticky traps is difficult. Therefore, a fast and accurate identification method is required. Here we analyzed barcode sequence variation based on two mitochondrial gene regions, cytochrome c oxidase subunit I (mtCOI) and cytochrome b (cytb) as DNA barcode, and one endosymbiont gene (wsp Wolbachia gene) among the species in southern Iran. The results of pairwise genetic distance values showed that cytb and mtCOI genes had the highest inter-specific variability. Based on the results, two species P. machinosus and P. repens which are present on one host plant together probably have been infected with similar Wolbachia strain.

کلیدواژه‌ها [English]

  • species identification
  • cytochrome c oxidase subunit I (mtCOI)
  • cytochrome b (cytb)

مراجع

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