بررسی خواص حشره کـشی نانو امولسیون اسانس پونه، (Mentha longifolia L. (Lamiaceae علیه سوسـک چهار نقطه ای حبوبات، (Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae

نوع مقاله : مقاله کامل، فارسی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

2 موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران.

چکیده

امروزه نانو امولسیون­ها به دلیل خواص فیزیکی و شیمیایی منحصر به فردی مانند سایز کوچک ذرات، ثبات فیزیکی طولانی مدت و شفافیت مورد توجه بسیاری از محققین قرار گرفته ­اند. در این تحقیق سمیت تنفسی اسانس پونه Mentha longifolia و نانوامولسیــون آن علیه سوسک چهارنقطــه ­ای حبــوبات Callosobruchus maculatus مطالــعه شد. آزمایش­ها در شرایط آزمایشگاهی، دمای 2±27 درجه سلسیوس، رطوبت نسبی 5±65 و در تاریکی انجام شد. اسانس پونه در ساعات اولیه پس از انجام آزمایش سمیت تنفسی بالاتری نسبت به نانوامولسیون داشت. در مرحله حشره کامل مقدار LC50 اسانس و نانوامولسیون به ترتیب برابر با 1/10 و 1/16 میکرولیتر بر لیتر هوا برآورد شد. مقدارLC50  در مرحله جنینی برای اسانس برابر با 9/4 میکرولیتر بر لیتر هوا و برای نانو امولسیون 4/9 میکرولیتر بر لیتر هوا تعیین شد. در غلظت 25 میکرولیتر بر لیتر هوا، میزان LT50 اسانس و نانوامولسیون به ترتیب برابر با 2/2 و 1/11 روز بود. در غلظت 1/10 میکرولیتر بر لیتر هوا اسانس معمولی دوامی نداشت، ولی نانوامولسیون دوام خوبی (روز 04/9 =LT50) داشت. در غلظت 9 میکرولیتر بر لیتر هوا مدت زمانی که طول کشید 50 درصد تخم­ها در اثر اسانس معمولی دچار مرگ ­و ­میر شوند، 7/3 روز برآورد شد. میزان LT50 نانوامولسیون در این غلظت 09/17 روز بود. در غلظت 9/4 میکرولیتر بر لیتر هوا میزان LT50 نانوامولسیون 8/12 روز بود، در حالیکه اسانس معمولی فاقد دوام بود. میانگین قطر ذرات نانوامولسیون با استفاده از میکروسکوپ الکترونی (TEM) حدود 36-10 نانومتر تعیین شد. بر اساس نتایج به دست آمده، بکارگیری فرم نانوامولسیون اسانس گیاه پونه می­تواند باعث افزایش سمیت تنفسی و دوام آن شود.

کلیدواژه‌ها

عنوان مقاله [English]

Study on insecticidal properties of nanoemulsion Mentha longifolia L. (Lamiaceae) essential oil against Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae)

نویسندگان [English]

  • M. Louni 1
  • Jahanshir Shakarami 1
  • Maryam Negahban 2
1 Department of Plant protection, Faculty of Agriculture Science, University of Lorestan, Khorramabad, Iran
2 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organi-zation (AREEO), Tehran, Iran
چکیده [English]

Nowadays, nanoemulsions are considered by many researchers because of their unique physiochemical properties such as small particle size, long-term physical stability and transparency. In this research, fumigant toxicity of Mentha longifolia L. essential oil and its nanoemulsion against Callosobruchus maculatus (Fabricius) has been studied. The experiments were conducted at laboratory conditions at 27±2°C temperature, 65±5% relative humidity and darkness. Essential oil of M. longifolia had higher fumigant toxicity than nanoemulsion, during early hours after treatment. The LC50 values of oil and nanoemulsion for the adults of the pest were 10.1 and 16.1 µl/l, respectively. The values of LC50 were 4.9 and 9.4 µl/l for oil and nanoemulsion during incubation period, respectively. In concentration of 25 µl/l, LT50 of M. longifolia essential oil and nanoemulsion were 2.2 and 11.1 days, respectively. In concentration of 10.1 µl/l, essential oil did not have durability, but this parameter of nanoemulsion is good (LT50= 9.04 days). At 9 µl/l of essential oil, the average time for the mortality of 50% eggs was 4.7 days. The LT50 of nanoemulsion at this concentration was 17.09 days. At 4.9 µl/l of nanoemulsion, the LT50 was 12.8 days, while essential oil did not have durability. The average diameter of nanoemulsion particles has been estimated about 10-36 nm by using transmission electron microscope (TEM). By using laser light scattering (SEMATech), this value was 234 nm. Considering obtained results, using nanoemulsion form of M. longifolia essential oil increase its fumigant toxicity and durability.

کلیدواژه‌ها [English]

  • Callosobruchus maculatus
  • Essential oil
  • Insecticidal
  • Mentha longifolia
  • Nanoemulsion

مراجع

Abbott, W. S. (1925) A method for computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265–267.
Allahvaisi, S., Talebi Jahromi, K., Imani, S. & Khanjani, M. (2017) Efficacy of electrospun bionanofibers as fumigant pesticides in foodstuff storage. Journal of Plant Protection Research 57, 72-80.
Dent, D. (2000) Insect pest management. Second edition. CABI Publishing, Ascot, UK.
Devi, M.B. & Devi, N.V. (2014) Biology and morphometric measurement of cowpea weevil, Callosobruchus maculatus fabr. (Coleoptera: Chrysomelidae) in green gram. Journal of Entomology and Zoology Studies 2, 74-76.
Emamjomeh, L., Imani, S., Talebi, Kh., Moharramipour, S. & K. Larijani, K. (2017) Preparation of nanoemulsion formulation of essential oil of Zataria multiflora and comparison of contact toxicity with pure essential oil on Ephestia kuehniella. Entomology and Phytopathology 85, 181-190.
Gusmao, N. M. S., Oliveira, J. V., Navarro, D. M. A. F., Dutra, K. A., Silva, W. A. & Wanderley, A. J. A. (2013) Contact and fumigant toxicity and repellency of Eucalyptus citriodora Hook., Eucalyptus staigeriana F., Cymbopogon winterianus Jowitt and Foeniculum vulgare Mill. Essential oils in the management of Callosobruchus maculatus (FABR.) (Coleoptera: Chrysomelidae, Bruchinae). Journal of Stored Products Research 54, 41-47.
Jamal, M., Moharramipour, S., Zandi, M. & Negahban, M. (2013) Efficacy of nanoencapsulated formulation of essential oil from Carum copticum seeds on feeding behavior of Plutella xylostella (Lep.: Plutellidae). Journal of Entomological Society of Iran 33, 23-31.
Khademi, N., Moharramipour, S. & Negahban, M. (2014) Insecticidal properties of essential oils of Carum copitum and Cuminum cyminum and their formulations on Sitophilus oryzae and Tribolium castaneum. M. Sc. thesis, Faculty of Agriculture, Tarbiat Modares University. 108 pp.
Liu, X. C., Lu, X. N., Liu, Q. Z. & Liu, Z. L. (2014) Evaluation of insecticidal activity of the essential oil of Allium chinense G. Don and its major constituents against Liposcelis bostrychophila Badonnel. Journal of Asia-Pacific Entomology 17, 853-856.
Majeed, H., Bian, Y.Y., Ali, B., Jamil, A., Majeed, U., Farid, Q., Iqbal, K.J., Shoemaker, C.F. & Fang, ZH. (2015) Essential Oil Encapsulations: Uses, Procedures, and Trends. The Royal Society of Chemistry 5, 58449-58463.
Moharramipour, S. & Negahban, M. (2014) Basic and applied aspects of biopesticides. Plant Essential Oil and Pest Management 129-155.
Moretti, M.D.L., Sanna-Passino, G., Demontis, S. & Bazzoni, E. (2002) Essential oil formulations useful as a new tool for the insect pest control. American Association of Pharmaceutical Scientists 3, 1-11.
Motamedi, Y., Fallahzadeh, M. & Roshan V. (2011) Contact toxicity of three plant essential oils on adult insects of Sithophilus oryzae (Coleoptera: Curculionidae). Plant Protection Journal 3, 135-144.
Negahban, M., Moharramipour, S. & Sefidkon, F. (2007) Fumigant toxicity of essential oil from Artemisia sieberi Besser against three stored-product insects. Journal of Stored Products Research 43, 123-128.
Negahban, M. (2012) Insecticidal propertises of nanocapsulated essential oil of Artemisia sieberi Besser on Plutella xylostella and Tribolium castaneum. Ph.D. thesis, Faculty of Agriculture, Tarbiat Modares University. 173 pp.
Negahban, M., Moharramipour, S., Zandi, M. & Hashemi S. A. (2013a) Efficiency of nanoencapsulated essential oil of Artemisia sieberi Besser on nutritional indices of Plutella xylostella. Iranian Journal of Medicinal and Aromatic Plants 29, 692-708.
Negahban, M., Moharramipour, S., Zandi, M. & Hashemi S. A. (2013b) Repellent activity of nanoencapsulated essential oil of Artemisia sieberi Besser on Plutella xylostellaL. larvae. Iranian Journal of Medicinal and Aromatic Plants 29, 909-924.
Nuchuchua, O., Usawadee, S., Uawongyart, N., Puttipipatkhachorn, S., Soottitantawat, A. & Ruktanonchai U. (2009) In Vitro Characterization and Mosquito (Aedes aegypti) Repellent Activity of Essential-Oils-Loaded Nanoemulsions. American Association of Pharmaceutical Scientists 10, 1234-1242.
Passino, G.S., Bazzoni, E. & Moretti, M. D. L. (2004) Microcapsulated essential oils active against Indian meal moth. Boletin de Sanidad Vegetal Plagas 30, 125-132.
 Ranjan, S., Dasgupta, N. & Lichtfouse, E. (2016) Nanoscience in Food and Agriculture. Sustainable Agriculture Reviews 20, 1-341.
Saeidi, M. & Moharramipour S. (2013) Insecticidal and repellent activities of Artemisia khorassanica, Rosmarinus officinalis and Mentha longifolia essential oils on Tribolium confusum. Journal of crop protection 2, 23-31.
SAS Institute, (1996). SAS/STAT user’s guide. Release 6.12 edition. Cary, NC.
Shahmirzaei, Z., Izadi, H. & Imani, S. (2016) Study on the contact and fumigant toxicity of Mentha longifolia L. against the confused flour beetle (Tribolium castaneum). Iranian Journal of Medicinal and Aromatic Plants 32, 556-559.
Shakarami, J., Pourhosseini, L., Vafaei-Shoushtari, R. & Goldasteh, S. (2008) Ovicidal effect of three plant essential oils on Callosobruchus maculatus F. (Col., Bruchidae). Journal of Entomological Research 3, 221-228.
Sharopov, F. S. (2012). Essential oil composition of Mentha longifolia from wild populations growing in Tajikistan. Journal of Medicinally Active Plants 1, 76-84.
Topuz, O. K., Ozvural, E. B., Zhao, Q., Huang, Q., Chikindas, M. & Gulukcu, M. (2016) Physical and antimicrobial properties of anise oil loaded nanoemulsions on the survival of foodborne pathogens. Food Chemistry 203, 117-123.
Ziaee, M., Moharramipour, S., & Mohsenifar, A. (2014) Toxicity of Carum copitum essential oil-loaded nanogel against Sitophilus granarius and Tribolium confusum. Journal of Applied Entomology 138, 763-771.
 
 

فایل ها

هم رسانی

ارجاع به این مقاله

آمار