Evaluation of COI and ITS2 sequences ability to distinguish Lysiphlebus fabarum and Lysiphlebus confusus (Hymenoptera: Aphidiidae)

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Abstract

All wasp species in the genus Lysiphlebus Förster are solitary endoparasitoids of aphids and
considered a taxonomically difficult group. In this study a part of COI and ITS2 was used to distinguish
two closely related species L. fabarum (Marshall) and L. confusus Tremblay & Eady in Iran. The
sequencing with a similarity about 99.5% showed COI's inability to separate the species L. fabarum and
L. confusus. The ITS2 region of the rDNA partly separated the species with the similarity of 96.5-98.5%.
The result is indicative of DNA barcoding deficiency in the identification of the species, as the two gene
regions fail to efficiently separate the species.

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Journal of Entomological Society of Iran
Volume 31, Issue 2
March 2012
Pages 53-66

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