Lethal toxicity of Achillea millefolium and Marrubium vulgare extracts, against potato tuber moth, Phthorimaea operculella (Lepidotera, Gelechiidae) in comparison with some synthetic pesticides

Document Type : Paper, Persian

Authors

1 Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran

2 Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.

Abstract

Nowadays, use of botanical compounds as pesticides is becoming increasingly important, because of their acceptable efficiency and relative safety to human and environment. The present study aimed to investigate oral toxicity of different extracts of Achillea millefolium L. and Marrubium vulgare L., against larvae of potato tuber moth and compare their potency with some conventional insecticides. Larvae of potato tuber moth, reared on potato tubers in controlled conditions. Extraction was carried out by maceration method using hexane, ethyl acetate, methanol and water as solvents. Foliage of A. millefolium and M. vulgare collected, well washed with distilled water, air dried in shadow then used for extraction. For bioassays fresh potato leaves dipped in each concentration of extracts for 5 seconds, air dried for 20 minutes. Potato tuber moth larvae transferred on treated leaves. Mortality was recorded after 48 hours. The results revealed that, aqueous and ethyl acetate extracts of A. millefolium showed the most and the least toxicity against 1st larval instar, with LC50 values of 2.96 and 44.03 gr/lrespectively. Methanol extract of A. millefolium and ethyl acetate extracts of M. vulgare were more and less toxic against 2nd larval instar of PTM with LC50 values of 9.03 and 34.79 gr/l, respectively. The toxicity comparison of plants extracts with Fenvalerate, Imidacloprid and Acetamiprid showed that, in all cases the synthetic pesticides were more toxic than plant extracts. Acetamiprid with LC50 values of 0.18 and 0.31 gr/l was more toxic than other pesticides against 1st and 2nd larval instars of PTM, respectively but relative potency of plant extracts was comparable with Fenvalerate.

Keywords

References

Abarca-Vargas, R., Peña Malacara, C. F. & Petricevich, V. L. (2016) Characterization of Chemical Compounds with Antioxidant and Cytotoxic Activities in Bougainvillea x buttiana Holttum and Standl, (var. Rose) Extracts. Antioxidants 5, 1-11.
Abbott, W. S. (1925) A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265-267.
Abd El-Aziz, M. F. (2011) Bioactivities and biochemical effects of Marjoram essential oil used against potato tuber moth Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae). Life Science Journal 8, 288-297.
Abegunde S. M. & Ayodele-Oduola R. O. (2015) Comparison of Efficiency of Different Solvents used for the Extraction of Phytochemicals from the Leaf, Seed and Stem Bark of Calotropis Procera. International Journal of Science and Research 4, 835-838.
Alkan, M., Gokce, A. & Kara K. (2017) Contact Toxicity of Six Plant Extracts to Different Larval Stages of Colorado Potato Beetle (Leptinotarsa decemlineata Say (Col: Chrysomelidae). Journal of Agricultural Sciences 23, 309-316.
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D. G. & Lightfoot, D. A. (2017) Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants 6, 1-23.
Amel, A. & Selima, B. (2015) Larvicidal effect of Marrubium vulgare on Culex pipiens ineastern Algeria. Energy Procedia 74, 1026 – 1031.
Amini Jam, N., Kocheyli, F., Mossadegh, M. S., Rasekh, A. & Saber, M. (2014) Lethal and sublethal effects of imidacloprid and pirimicarb on the melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae) under laboratory conditions. Journal of Crop Protection 3, 89-98.
Aouati, A. & Berchis, B. (2015) Larvicidal effect of Marrubium vulgare on Culex pipiens in eastern Algeria. Energy Procedia 74, 1026 – 1031.
Azwanida, N. N. (2015) A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants 4, 1-6.
Barile, F. A. (2008) Principals of Toxicology Testing. 312 pp. CRC Press. New York.
Briese, D. T. (1986) Geographic variability in demographic performance of the potato moth, Phthorimaea operculella Zell. In Australia. Bulletin of Entomological Research 76, 719–726.
Çakır, A., Özer, H., Aydın, T., Kordali, Ş., Tazegül, Çavuşoglu, A., Akçin, T., Mete, E. & Akçin, A. (2016) Phytotoxic and insecticidal properties of essential oils and extracts of four Achillea species. Records of natural products 10, 154-167.
Chandel, R. S., Bhatia, D., Singh, B. & Chandla. V. K. (2010) Susceptibility of potato tuber moth, Phthorimaea operculella (zeller) to CIPC (chlorpropham). Potato Journal 37, 121-130.
Dailey, A. & Vuong, Q. V. (2015) Effect of extraction solvents on recovery of bioactive compounds and antioxidant properties from macadamia (Macadamia tetraphylla) skin waste. Cogent Food & Agriculture 1, 1-10.
Das, G. P. (1995) Plants used in controlling the potato tuber moth, Phthorimaea operculella (Zeller). Crop Protection 14, 631-636.
Dhawan, D. & Gupta, J. (2017) Comparison of different solvents for phytochemical extraction potential from Datura metel Plant Leaves. International Journal of Biological Chemistry 11, 17-22.
Dimetry, N. Z. (2012) Prospects of botanical pesticides for the future in integrated pest management program (IPM) with special reference to neem uses in Egypt. Archives of Phytopathology and Plant Protection 45, 1138-1161.
 Dinse, G. E. &  Umbach, D. M. (2013) Quantifying relative potency in dose-response studies. pp. 315-331 In  Ting Lee, M. L., Gail, M., Pfeiffer, R. &  Satten, G. A. (Eds), Risk assessment and evaluation of predictions. Springer USA.
Dubey, N. K., Srivastava, B. & Kumar, A. (2008) Current status of plant products as botanical pesticides in storage pest management. Journal of Biopesticides 1, 182–186.
Enyiukwu, D. N., Ononuju, C. C. Awurum, A. N. & Nwaneri, J. A. (2016) Modes of action of potential phyto-pesticides from tropical plants in plant health management. IOSR Journal Of Pharmacy 6, 1-17.
El-Wakeil, N. E. (2013) Botanical pesticides and their mode of action. Gesunde Pflanzen 65,125–149.
Felhi, S., Dadud, A., Hajladui, H., Mnafgui, K., Gharsallah, N. & Kadri, A. (2017) Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits. Food Science and Technology 37, 483-492.
Fenemore, P. G. (1988) Host-plant location and selection by adult potato moth, Phthorimaea operculella (Lepidoptera: Gelechiidae): A review. Journal of Insect Physiology 34, 175–177.
Ferreira Maia, M. & Moore, S. J. (2011) Plant-based insect repellents: a review of their efficacy, development and testing. Malaria Journal 10(Suppl 1): 1-15.
Gamil, W. E., Mariy, F. M, Youssef, L. A. & Abdel Halim S. M. (2011) Effect of Indoxacarb on some biological and biochemical aspects of Spodoptera littoralis (Boisd.) larvae. Annals of Agricultural Science 56, 121–126.
Gökçe, A., Whalon, M. E. Çam, H. Yanar, Y. Demirtaş, I. & Goren, N. (2006) Plant extract contact toxicities to various developmental stages of Colorado potato beetles (Coleoptera: Chrysomelidae).  Annals of Applied Biolog 149, 197–202.
Gottschalk, P. G. & Dunn, J. R. (2005) Measuring parallelism, linearity, and relative potency in bioassay and immunoassay data. Journal of Biopharmaceutical Statistics 15, 437–463.
Gould, F. (1991) Arthropod Behavior and the Efficacy of Plant Protectants. Annual Review of Entomology 36, 305-330.
Granados-Echegoyen, C., Pérez-Pacheco, R., Bautista-Martínez, N., Alonso-Hernández, N., Guerra, P. C., Molina, I. Y., Yabar, E. & Gianoli, E. (2007) Oviposition deterrence of shoots and essential oils of Minthostachys spp. (Lamiaceae) against the potato tuber moth. Journal of Application Entomology 131, 134–138.
Hannour, K., Boughdad, A., Maataoui, A. & Bouchelta, A. (2017) Chemical composition and toxicity of Moroccan Rosmarinus officinalis (Lamiaceae) essential oils against the potato tuber moth, Phthorimaea operculella (Zeller, 1873) Zeller (Lepidoptera, Gelechiidae). Journal of materials and Environmental Sciences 8, 758-769.
Hasheminia, S. M., JalaliSendi, J., Talebi Jahromi, K. & Moharramipour, S. (2011) The effects of Artemisia annua L. and Achillea millefolium L. crude leaf extracts on the toxicity, development, feeding efficiency and chemical activities of small cabbage Pieris rapae L. (Lepidoptera: Pieridae). Pesticide Biochemistry and Physiology 99, 244-249.
Hikal, W. M., Baeshen, R. S. & Said-Al Ahl, H. (2017) Botanical insecticide as simple extractives for pest control, Cogent Biology 3, 1-16.
Isman, M. B. (2006) Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51, 45–66.
Kchaou, W., Abbès, F., Blecker, C., Attia, H. & Besbes S. (2013) Effects of extraction solvents on phenolic contents and antioxidant activities of Tunisian date varieties (Phoenix dactylifera L.),  Industrial Crops and Products 45, 262–269.
Koul, O., Walia, S. & Dhaliwal, G. S. (2008) Essential oils as green pesticides: potential and constraints. Biopesticide International 4, 63-84.
Laxmishree, C. & Nandita, S. (2018) Review of Mode of action of some major botanical pesticides. Int. Research Journal of Science & Engineering 6, 129-132.
Laxmishree, C. & Nandita, S. (2017) Botanical pesticides - a major alternative to chemical pesticides: A review. International Journal of Life Sciences 5, 722-729.
Lei, C. & Sun, X. (2018) Comparing lethal dose ratios using probit regression with arbitrary slopes. BMC Pharmacology and Toxicology 19, 1-10.
Lodhi, S., Vadnere, G. P., Sharma, V. K. & Usman, M. R. (2017) Marrubium vulgare L.: A review on phytochemical and pharmacological aspects. Journal of Intercultural Ethnopharmacology 6,429-452.
Lushchak, V. I., Matviishyn, T. M., Husak, V. V., Storey, J. M. & Storey, K. B. (2018) Pesticides toxicity: a mechanistic approach.  EXCLI Journal 17:1101-1136.
Mahin Allahverdizade, N. & Mohammadi, D. (2016) Bioactivity of Marrubium vulgare and Achillea millefolium leaf extracts on potato tuber moth Phthorimaea operculella Zeller. Munis Entomology and Zoology 1, 114-122.
Matsumura, F. (1985) Toxicology of Insecticides. 2nd ed. 598 pp. Plenum Press, New York and London.
Nakhaie Bahrami, M., Mikani, A. & Moharramipour, S. (2018) Effect of Achillea millefolium and Teucrium polium extracts on nutritional indices and α-amylase and protease activities of Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae). Journal of Crop Protection 7, 183-190.
Niroula, S. P. & Vaidya, K. (2004) Efficacy of some botanicals against potato tuber moth, Phthorimaea operculella (Zeller, 1873). Our Nature 2, 21-25.
Orhana, I. E., Belhattabb, R., Şenola, F. S., Gülpinarc, A. R., Hoşbaş, S. & Kartalc M. (2010) Profiling of cholinesterase inhibitory and antioxidant activities of Artemisia absinthium, A. herba-alba, A. fragrans, Marrubium vulgare, M. astranicum, Origanum vulgare subsp. glandulossum and essential oil analysis of two Artemisia species. Industrial Crops and Products 32, 566-571.
Paramasivam, M. & Selvi, C. (2017) Laboratory bioassay methods to assess the insecticide toxicity against insect pests-A review. Journal of Entomology and Zoology Studies 5, 1441-1445.
Prabhaker, N., Castle, S. J. & Toscano, N. C. (2006) Susceptibility of Immature Stages of Homalodisca coagulate (Hemiptera: Cicadellidae) to Selected Insecticides. Journal of Economic Entomology 99, 1805-1812.
Prakash, A., Rao, J. & Nandagopal, V. (2008) Future of botanical pesticides in rice, wheat, pulses and vegetables pest management. Journal of Biopesticides 1,154–169.
Rafiee-Dastjerdi, H., Khorrami, F., Razmjou, J., Esmaielpour, B., Golizadeh, A. & Hassanpour, M. (2013a) The efficacy of some medicinal plant extracts and essential oils against potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae). Journal of Crop Protection 2, 93-99.
Rafiee-Dastjerdi, H., Mashhadi, Z. & Sheikhi Garjan, A. (2013b) Lethal and sublethal effects of abamectin and deltamethrin on potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae). Journal of Crop Protection 2, 403-409.
Rattan, R. S. (2010) Mechanism of action of insecticidal secondary metabolites of plant origin. Crop Protection 29, 913-920.
Regnault-Roger, C. (1997) The potential of botanical essential oils for insect pest control. Integrated Pest Management Reviews 2, 25–34.
Rodriguez-Saona, C., Wanumen, A. K., Salamanca, J., Holdcraft, R. & Kyryczenko-Roth, V. (2016) Toxicity of insecticides on various life stages of two Tortricid pests of Cranberries and on a non-target predator. Insects 7, 1-20.
Rondon, S. I. (2010) The potato tuber worm: a literature review of its biology, ecology, and control. American Journal of Potato Research 87,149–166.
Rondon, S. I., DeBano, S. J., Clough, G. H., Hamm, P. B., Jensen, A., Schreiber, A., Alvarez, J. M., Thornton, M., Barbour, J. & Dŏgramaci, M. (2007) Biology and management of the potato tuberworm in the Pacific Northwest. A Pacific Northwest Extension publication 594, 1-7.
Rozman, V. (2015) Control of stored products pests by natural products. Integrated Protection of Stored Products 111, 295-299.
Sánchez-García, J. A., Martinez-Tomas, S. H. & Sánchez-Mendoza, S. (2015) Insecticidal effect of botanical extracts on developmental stages of Bactericera cockerelli (Sulc) (Hemiptera: Triozidae). Southwestern Entomologist 40, 97-110.
Sevindik, H. G., Güvenalp, Z., Yerdelen, K. O., Yuca, H. & Demirezer, L. O. (2015) The discovery of potential anticholinesterase compounds from Achillea millefolium L. Industrial Crops and Products 76, 873-879.
Sharaby, A., Abdel-Rahman, H. & Moawad, S. (2009) Biological effects of some natural and chemical compounds on the potato tuber moth, Phthorimaea operculella Zell. (Lepidoptera:Gelechiidae). Saudi Journal of Biological Sciences 16, 1–9.
Shelton, A. M. & Wyman, J. A. (1979) Potato tuber worm damage to potato grown under different irrigation and cultural practices. Journal of Economic Entomology 72, 261–264.
Sisay, A. & Ibrahim, A. (2012) Evaluation of some potential botanicals to control potato tuber moth, (Phthorimaea Operculella) under storage condition at Bako, western Ethiopia. ESci journal of plant pathology 1, 14-18.
Siyahlou, S., Barzin, G. & Entezari, M. (2016) Review and study of Achillea milefollium essential oil compounds in various stages of development. International Research Journal of Applied and Basic Sciences 10, 1112-1115.
SPSS Inc. Released (2007) SPSS for Windows, Version 16.0. Chicago, SPSS Inc.
Stamopoulos, D. C., Damos P. & Karagianidou, G. (2007) Bioactivity of five monoterpenoid vapours to Tribolium confusum (du Val) (Coleoptera: Tenebrionidae). Journal of Stored Product Research 43, 571-577.
Stenersen, J. (2004) Chemical pesticides: mode of action and toxicology. CRC Press. New York Washington, D.C. 276 pp.
Trivedi, A., Nayak, N. & Kumar, J. (2018) Recent advances and review on use of botanicals from medicinal and aromatic plants in stored grain pest management. Journal of Entomology and Zoology Studies 6, 295-300.
Varela, L. G. & Bernays, E. A. (1988) Behavior of newly hatched potato tuber moth larvae, Phthorimaea operculella Zell. (Lepidoptera: Gelechiidae), in relation to their host plants. Journal of Insect Behavior 1, 261-275.
Zlotek, U., Mikulska, S., Nagajek, M. & Swieca, M. (2016) The effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) extracts. Saudi Journal of Biological Sciences 23, 628–633. 

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