Document Type : Paper, Persian
Authors
1
Plant Protection Research Department, Khuzestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran
2
Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
10.61186/jesi.45.3.12
Abstract
Successful mass rearing of Telenomus busseolae Gahan relies on the large-scale production of Sesamia spp. eggs, in Iran. The present study aimed to investigate the effect of artificial substrates (filter paper, parafilm, wax paper, and cellophane) in both dry and wet form, on the oviposition of S. cretica Lederer and S. nonagrioides Lefebvre as the main treatments, with and without an oviposition stimulant compared to sugarcane stem (shoot) as the control treatment, across 20 replications in a completely randomized design. Each artificial substrate was soaked with 1 ml. of corn leaf extract (oviposition stimulant) and placed in a container of water. All experiments were conducted at 27±1°C, 50±10% RH, and a photoperiod of 16:8 h (L:D). Based on the results obtained, the highest egg-laying for S. cretica and S. nonagrioides were observed in natural host (sugarcane shoot) with 156.92± 5.934 and 324.89± 15.274 eggs per female, respectively. Two artificial substrates, moist cellophane and parafilm (with the oviposition stimulant), were grouped together in terms of fecundity, following the natural host treatment with 71.3± 2.019 and 64.95±1.609 eggs per female in S. cretica and 142.6± 3.260 and 136.65± 3.546 eggs per female in S. nonagrioides, respectively. The cellophane substrate, due to its lower adhesion, easier application, and lower cost compared to parafilm, is more efficient egg-laying substrate for moths. Overall, despite the problems of using natural substrates, for the mass rearing of Sesamia spp. in insectarium, the use of the sugarcane shoots during mating and egg-laying stages is superior to artificial substrates. However, if access to natural host shoots, was not possible, especially in laboratory studies, a moist cellophane substrate with corn leaf extract would serve as a suitable alternative. The results of this research can facilitate the mass rearing of Sesamia spp. under laboratory condition and in insectariums.
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