تأثیر گیاه توتون تراریخته، Nicotiana tabacum حاوی ژن بازدارنده فعالیت ریبوزوم جدا شده از پیاز گیاه زنبق هلندی، Iris hollandica روی پارامترهای بیولوژیکی(Myzus nicotianae (Hem.: Aphididea و (Spodoptera exigua (Lep.: Nocuidae

نویسنده

گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ولی‌عصر(عج) رفسنجان، ایران.

چکیده

استفاده از گیاهان زراعی اصلاح شده با استفاده از انتقال ژن بین گونه‌های مختلف گیاهی نقش مهمی‌در کنترل آفات گیاهی داشته است. در این پژوهش، خواص حشره‌کشی دو لاین از گیاهان تراریخته شاملIRIP  (حاوی ژن بازدارنده ریبوزوم نوع 1) و IRA (حاوی ژن بازدارنده ریبوزوم نوع 2) علیه کرم برگخوار چغندرقندSpodoptera exigua Hübner (Lepidoptera: Noctuidae) و شته توتون،Blackman (Hemiptera: Aphididae)  Myzus nicotianae بررسی شده است. تغذیه از گیاه توتون تراریخته IRA، باعث افزایش مرگ­ومیر و کاهش تولید مثل در شته توتون شد. هم‌چنین طول دوره تولیدمثلی شته‌های پرورش یافته روی گیاه IRA در مقایسه با تیمار شاهد و IRIP کاهش یافت. اما پارامترهای تولیدمثلی شته‌های تیمار شده باIRIP  تفاوت معنی­داری با شاهد نداشتند. تغذیه لاروهای سن دو exigua . Sاز گیاه تراریخته IRA، باعث مرگ­ومیر حدود 3/33 درصد شد. به­علاوه وزن لاروها و شفیره‌ها و درصد خروج حشرات کامل S. exigua نیز در تیمار  IRA نسبت به شاهد و تیمار گیاهان تراریخته  IRIPکاهش معنی­داری نشان دادند. نتایج حاکی از این است که پروتئین‌های بازدارنده فعالیت ریبوزوم نوع دوم دارای فعالیت حشره‌کشی قابل­توجهی روی هر دو گونه حشره آفت می­باشند که می‌توانند در برنامه‌های کنترل تلفیقی آفات به­کار گرفته شوند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of transgenic tobacco plant, Nicotiana tabacum expressing ribosome inactivating gene isolated from the bulbs of Dutch iris, Iris hollandica on biological parameters of Myzus nicotianae (Hem.: Aphididea) and Spodoptera exigua (Lep.: Noctuidae)

نویسنده [English]

  • Sh. Shahidi-Noghabi
Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.
چکیده [English]

The use of modified crop plants through gene transfer between different plant species plays an important role in pest control programs. We have investigated the insecticidal properties of two lines of tobacco transgenic plant consisting of IRIP (expressed with type 1 ribosome inactivating gene) and IRA (expressed with type 2 ribosome inactivating gene) against beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae) and tobacco aphid, Myzus nicotianane Blackman (Hemiptera: Aphididae). The transgenic tobacco plants expressing IRA increased mortality and decreased reproduction rate of tobacco aphid. The reproductive period of tobacco aphid fed on transgenic tobacco plant expressing IRA reduced in comparison with the control and IRIP. We did not observed any impact on reproduction parameters of the aphids treated with IRIP plant in comparison with control. Feeding second instar larvae of S. exigua on transgenic tobacco plant expressing IRA led to about 33.3% mortality. Weight of larvae and pupae as well as the rate of the emergence of adult S. exigua were significantly decreased in larvae fed on IRA in comparison with control. These results suggest that type-2 ribosome inactivating proteins serve as significant insecticidal factors on both insect pest and can be used in integrated pest control programs.

کلیدواژه‌ها [English]

  • Lepidoptera
  • Ribosome inactivating proteins
  • Hemiptera
  • Lectins

مراجع

Barbieri, L., Ciani, M., Girbes, T., Liu, W. Y., Van Damme, E. J. M., Peumans, W. & Stripe, F. (2004) Enzymatic activity of toxic and non-toxic type 2 ribosome-inactivating proteins. FEBS Letters 563, 219-222.
Baykal, U. & Tumer, N. E. (2007) The C-terminus of pokeweed antiviral protein has distinct roles in transport to the cytosol, ribosome depurination and cytotoxicity. The Plant Journal49, 995-1007.
Blackman, R. L. & Eastop, V. F. (2000) Aphids on the World's Crops: An Identification and Information Guide: Wiley, John & Sons, Incorporated. 2 nd ed. 476 pp. The University of Michigan
Chen, Y., Peumans, W. J. & Van Damme, E. J. M. (2002) The Sambucus nigra type-2 ribosome-inactivating protein SNA-I' exhibits in planta antiviral activity in transgenic tobacco. FEBS Letters 516, 27-30.
Desmyter, S., Vandenbussche, F., Hao, Q., Proost, P., Peumans, W.J. & Van Damme, E. J. M. (2003) Type-1 ribosome-inactivating protein from iris bulbs: a useful agronomic tool to engineer virus resistance? Plant Molecular Biology 51, 567-576.
Dowd, P. F., Rober, A. H., Pinkerton, T. S., Johnson, E. T., Lagrimini, L. M. & Boston, R. S. (2006) Relative activity of a tobacco hybrid expressing high levels of a tobacco anionic peroxidase and maize ribosome-inactivating protein against Helicoverpa zea and Lasioderma serricorne. Journal of Agricultural and Food Chemistry 54, 2629-2634.
Dowd, P. F., Zou, W. N., Gillikin, J. W., Johnson, E. T. & Boston, R. S. (2003) Enhanced resistance to Helicoverpa zea in tobacco expressing an activated form of maize ribosome-inactivating protein. Journal of Agricultural and Food Chemistry 51, 3568-3574.
Gatehouse, A. M. R., Barbieri, L., Stirpe, F. & Croy, R. R. D. (1990) Effects of ribosome-inactivating proteins on insect development differences between Lepidoptera and Coleoptera. Entomologia Experimentalis et Applicata 54, 43-51.
Hakim, R. S., Blackburn, M., Corti, P., Gelman, D., Goodman, C., Elsen, K., Loeb, M., Lynn, D. & Smagghe, G. (2006) Growth and mitogenic effects of arylphorin in vivo and in vitro. Archives of insect Biochemistry and Physiology 64, 63-73.
Jouanin, L., Bottino, M., Girard, C., Morrot, G. & Giband, M. (1998). Transgenic plants for insect resistance. Plant Science 131, 1-11.
Metcalf, C. L., Flint, W. P. & Metcalf, R. L. (1962) Destructive and useful insects their habits and control. 4th ed. 1087pp. McGraw-Hill Book Co., New York.
Peumans, W. J., Barre, A., Hao, Q., Rougé, P. & Van Damme, E. J. M. (2000) Higher plants developed structurally different motifs to recognize foreign glycans. Trends in Glycoscience and Glycotechnology 12, 83-101.
Peumans, W. J., Hao, Q. & Van Damme, E. J. M. (2001) Ribosome-inactivating proteins from plants: more than RNA N-glycosidases? FASEB Journal 15, 1493-1506.
Ranjekar, P. K., Patankar, A., gupta, V., Bhatnagar, R., Bentur, J. & Kumar, P. A. (2003). Genetic engineering of crop plants for insect resistance. Current Science 84, 321-29.
Stirpe, F. & Battelli, M. G. (2006) Ribosome-inactivating proteins: progress and problems. Cellular and Molecular Life Sciences 63, 1850-66.
Senthil-Nathan, S., Choi, M. Y., Paik, C. H. & Kalaivani, K. (2008) The toxicity and physiological effect of goniothalamin, a styryl-pyrone, on the generalist herbivore, Spodoptera exigua Hubner. Chemosphere 72, 1393-1400.
Shahidi-Noghabi, S., Van Damme, E. J. M., Iga, M. & Smagghe, G. (2010a) Exposure of insect midgut cells to Sambucus nigra L. agglutinins I and II causes cell death via caspase-dependent apoptosis. Journal of Insect Physiology56, 1101-1107.
Shahidi-Noghabi, S., Van Damme, E. J. M., De Vos, W. H., Smagghe, G. (2011) Internalization of Sambucus nigra agglutinins I and II in insect midgut cells. Archives of Insect Biochemistry and Physiology 76, 211-222.
Shahidi-Noghabi, S., Van Damme, E. J. M., Mahdian, K. & Smagghe, G. (2010b)Entomotoxic action of Sambucus nigra agglutinin I in Acyrthosiphon pisum aphids and Spodoptera exigua caterpillars through caspase-3 like dependent apoptosis. Archives of Insect Biochemistry and Physiology 75, 207-220.
Shahidi-Noghabi, S., Van Damme, E. J. M. & Smagghe, G. (2009) Expression of Sambucus nigra agglutinin (SNA-I′) from elderberry bark in transgenic tobacco plants results in enhanced resistance to different insect species. Transgenic Research 18, 249-259
Smagghe, G., Pineda, S., Carton, B., Del Estal, P., Budia, F. & Viñuela, E. (2003) Toxicity and kinetics for methoxyfenozide in greenhouse-selected Spodoptera exigua (Lepidoptera: Noctuidae). Pest Management Science 59, 1203-1209.
Steeves, R. M., Denton, M. E., Barnard, F. C., Henry, A. & Lambert, J. M. (1999) Identification of three oligosaccharide binding sites in ricin. Biochemistry 38, 11677-11685.].
Stirpe, F. (2004) Ribosome-inactivating proteins. Toxicon 44, 371-383.
Tamura, T., Sadakat, N., Oda, T. & Muramatsu, T. (2002) Role of zinc ions in ricin-induced apoptosis in U937 cells. Toxicology Letters 132, 141-151.
Van Damme. E. J. M., Hao, Q., Chen, Y., Barre, A., Vandenbussche, F., Desmyter, S., Rougé, P. & Peumans, W. J. (2001) Ribosome-inactivating proteins: a family of plant proteins that do more than inactivate ribosomes. Crit Rev. Plant Science. 20, 395-465.
Van Damme, E. J. M. (2008) Plant lectins as part of the plant defense system against insects. pp. 285-307 In: Schaller, A. (Ed.) Induced plant resistance to herbivory, Springer Science, Dordrecht, The Netherlands,
Van De Veire, M., Smagghe, G. & Degheele, D. (1997) Laboratory test method to evaluate the effect of 31 pesticides on the predatory bug, Orius laevigatus (Het.: Anthocoridae). Entomophaga 41, 235-244.
Vandenbussche, F., Peumans, W.J., Desmyter, S., Proost, P., Ciani, M. & Van Damme, E. J. M. (2004) The type-1 and type-2 ribosome-inactivating proteins from Iris confer transgenic tobacco plants local but not systemic protection against viruses. Planta 220, 211-221.
Wei, G. Q., Liu, R. S., Wang, Q. & Liu, W. Y. (2004) Toxicity of two type II ribosome-inactivating proteins (cinnamomin and ricin) to domestic silkworm larvae. Archives of Insect Biochemistry and Physiology 57, 160-165.
Zhou, X., Li, X. D., Yuan, J. Z., Tang, Z. H. & Liu, W. Y. (2000) Toxicity of cinnamomin-a new type II ribosome-inactivating protein to bollworm and mosquito. Insect Biochemistry and Molecular Biology 30, 259-264.
نامه انجمن حشره شناسی ایران
دوره 35، شماره 3
مهر 1394
صفحه 51-61

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