Tachykinin stimulates α-amylase, protease and lipase activities in the midgut of the American cockroach, Periplaneta americana (Blattodea: Blattidae)

نویسنده

دانشکده کشاورزی دانشگاه تربیت مدرس

چکیده

       در این تحقیق به بررسی اثر تاکیکینین بر روی فعالیت آلفا آمیلاز، پروتئاز و لیپاز در معده میانی سوسری امریکایی
(L.) Periplaneta americana پرداخته شد. فعالیت ایمنوهیستوشیمیایی سلول‌های معده میانی (بخش های جلویی، میانی و عقبی) که تاکیکینین ترشح می‌کردند مورد تایید قرار گرفت. چهار هفته گرسنگی، تعداد سلول هایی را که عکس العمل ایمنوهیستوشیمیایی مقابل آنتی‌بادی تاکیکینین داشتند (Tachykinin-ir cells) را کاهش داد، در حالی که تغذیه دوباره پس از سه ساعت Tachykinin-ir cells را افزایش داد. آزمون الایزا نیز نتیجه فوق را تایید کرد. قرار دادن معده میانی در بافر حاوی تاکیکینین منجر به افزایش فعالیت آلفا آمیلاز، پروتئاز و لیپاز شد در حالی که بافر به تنهایی هیچ اثری بر روی فعالیت آنزیم‌های فوق نداشت. تزریق تاکیکینین به همولنف حشره منجر به افزایش فعالیت آنزیم‌های فوق در معده میانی شد، در حالی که تزریق بافر هیچ تاثیری بر روی فعالیت آنزیم ها نداشت. به طور کلی می‌توان نتیجه گرفت که تغذیه باعث ترشح تاکیکینین از سلول های معده میانی شده که آن نیز به نوبه خود فعالیت آنزیم های آلفا آمیلاز، پروتئاز و لیپاز را افزایش می‌دهد. 

کلیدواژه‌ها

عنوان مقاله [English]

Tachykinin stimulates α-amylase, protease and lipase activities in the midgut of the American cockroach, Periplaneta americana (Blattodea: Blattidae)

نویسنده [English]

  • Azam Mikani
.
چکیده [English]

The effect of a neuropeptide, the tachykinin on α-amylase, protease and lipase activities in the midgut of American cockroach, Periplaneta americana (Linnaeus) is evaluated . Immunohistochemical reactivities against tachykinin (tachykinin- ir) were detected in the midgut. Tachykinin-ir cells in three regions (anterior, median and posterior) of the midgut were counted in normally fed, starved for four weeks and refed cockroaches. The number of tachykinin-ir cells decreased during 4 weeks of starvation and increased 3 h after refeeding. The results of the competitive ELISA were consistent with the profile of immunohistochemical reactivity. Incubation of the dissected midgut with tachykinin led to an increase in α-amylase, protease and lipase activities while the buffer remained ineffective. Tachykinin injection into the hemocoel increased α-amylase, protease and lipase activities, but PBS injection showed no sign of effectiveness. The results seggest that nutrients upregulate midgut cells secreting tachykinin causing further activities of digestive enzymes.

کلیدواژه‌ها [English]

  • Tachykinin
  • Digestive enzymes
  • Midgut
  • Periplaneta americana

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