ویژگی‌‎های بیوشیمیایی پروتئازهای گوارشی کفشدوزک خربزه Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)

نوع مقاله : مقاله کامل، فارسی

نویسندگان

1 هیات علمی / مرکز تحقیقات کشاورزی مازندران- تحقیقات گیاهپزشکی تنکابن

2 دانشجوی سابق کارشناسی ارشد گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان

3 گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان

چکیده

کفشدوزک خربزه، (Col.:Coccinellidae)Epilachna chrysomelina (Fabricius) به عنوان یکی از مهم­ترین آفات گیاهان خانواده کدوئیان قادر است با تغذیه از برگ‌های‌ گیاه در سنین لاروی و حشره کامل خسارت جدی به این محصولات وارد ‌سازد. در تحقیق حاضر ویژگی­های بیوشیمیایی پروتئازهای گوارشی در کفشدوزک خربزه مورد بررسی قرار گرفت. اندازه‌گیری فعالیت پروتئازی در لوله گوارش سنین 2، 3 و 4 لاروی و حشرات کامل نر و ماده نشان داد که بیش­ترین فعالیت آنزیم در لاروهای سن 3 بوده و فعالیت آن در حشرات نر و ماده اختلاف معنی­داری با هم ندارند. مقدارpH  و دمای بهینه فعالیت پروتئازی در لوله گوارش به­ترتیب 5 و 30 درجه سلسیوس بدست آمد. بازدارنده‌های EDTA،TLCK،TPCK،PMSF و یدواستات روی فعالیت پروتئازهای گوارشیE. chrysomelina  به ترتیب 93/18، 04/19، 27/22، 05/26 و 32/44 درصد مهارکنندگی نشان دادند. با توجه به بیشترین میزان بازدارندگی ایجاد شده توسط یدواستات روی فعالیت پروتئازی، سیستئین پروتئازها به عنوان پروتئازهای غالب در لوله گوارش این حشره معرفی می‌شوند. نتایج زایموگرام روی ژل نشان داد که آنزیم پروتئاز در لوله گوارشE. chrysomelina ، یک ایزوفرم دارد. 

کلیدواژه‌ها

عنوان مقاله [English]

Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)

نویسندگان [English]

  • Moloud Gholamzadeh-Chitgar 1
  • roohangiz Ghanbarinezhad 2
  • Mohammad Ghadamyari 3
1 Plant Protection Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran
2 Former student, department of plant protection, faculty of agricultural science, university of guilan
3 Department of Plant Protection, faculty of agricultural science, university of guilan
چکیده [English]

Melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)is a major pest of cucurbitaceous plants by feeding on leaves at larval and adult stages. In this study, biochemical properties of digestive proteases of the melon ladybird were studied in its alimentary canal. Enzyme activity in the digestive systems of 2nd, 3rd and 4th larval instars and adults showed that the highest specific enzyme activities occurred in 3rd instar larvae while no significant differences observed in adults. The optimal pH and temperature for protease activity in alimentary canal were found 5 and 30°C, respectively. The inhibitory effect of EDTA, TLCK, TPCK, PMSF and Iodoacetate were determined 44.32, 26.05, 22.27, 19.04 and 18.93% in the gut of E. chrysomelina, respectively. With respect to the highest inhibition rate in proteolytic activity caused by Iodoacetate, cysteine proteinases are considered as the main proteases in the midgut of the melon ladybird. Zymogram pattern in the native gel indicated that the protease enzyme had one isoform in the alimentary canal of the pest.

کلیدواژه‌ها [English]

  • melon ladybird
  • inhibitor
  • protease

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