سازوکار مقاومت به دلتامترین در جمعیت‌های مزرعه‌ای مینوز برگ گوجه‌فرنگی Tuta absoluta (Lepidoptera: Gelechiidae)

نوع مقاله : مقاله کامل، فارسی

نویسنده

گروه گیاهپزشکی- دانشکده کشاورزی- دانشگاه بوعلی سینا- همدان

چکیده

مینوز برگ گوجه­فرنگی Tuta absoluta (Meyrick) یک تهدید جدی برای تولید گوجه­فرنگی در شرایط مزرعه و گلخانه محسوب می­شود. تحقیق حاضر با هدف بررسی حساسیت جمعیت­های مزرعه­ای مینوز برگ گوجه­فرنگی به حشره­کش دلتامترین و تعیین سازوکار مقاومت با استفاده از سینرژیست­ها انجام شده است. زیست­سنجی به روش غوطه‌وری برگ در محلول سمی و روی لاروهای سن دوم انجام شد. نرخ مقاومت محاسبه شده بین 88/1 تا 58/21 برابر در مقایسه با سویه حساس متغیر بود. در آزمون­های سینرژیسمی، از PBO، DEF و DEM جهت بررسی احتمال دخالت سیستم­های آنزیمی در ایجاد مقاومت استفاده شد. برگ­های آغشته به غلظت­های مناسب از هر سینرژیست به مدت 12 ساعت قبل از شروع زیست­سنجی با حشره­کش، جهت تغذیه در اختیار لاروهای سن 2 قرار داده شد. بالاترین غلظت از هر سینرژیست که در جمعیت حساس هیچ گونه تلفاتی را همراه نداشت، برای سینرژیست­های PBO، DEF و DEM به ترتیب عبارت بودند از 100، 100 و 120 میلی­گرم بر لیتر. بر خلاف DEM و DEF، PBO، مهار کننده منواکسیژنازهای وابسته به سیتوکروم پی 450، به طور معنی­داری سمیت دلتامترین را در هر سه جمعیت مزرعه­ای افزایش داد (بالاترین نرخ سینرژیسم 44/5). به نظر می­رسد که در شرایط موجود، استفاده صرف از ترکیبات پایرتروییدی جهت کنترل مینوز برگ گوجه­فرنگی از جهت گسترش مقاومت به صلاح نباشد.

کلیدواژه‌ها


عنوان مقاله [English]

Mechanism of deltamethrin resistance in field populations of Tuta absoluta (Lepidoptera: Gelechiidae)

نویسنده [English]

  • M. Malekmohammadi
Dept. of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
چکیده [English]

The tomato leaf miner Tuta absoluta (Meyrick) may pose a threat to both greenhouse and open-field tomato production. The aims of this study were: 1) to assess the susceptibility to deltamethrin of
seven field populations of T. absoluta in Hamedan province, in comparison with susceptible strain. 2) to investigate the effects of synergists for testing possible mechanisms involved in resistance.
Bioassays were done by leaf dipping method to determine the resistance level. Resistance ratios
calculated varied from 1.9- to 21.6-times (compared with the susceptible population). In synergism tests, PBO, DEF, and DEM were used to determine whether metabolism was involved in deltamethrin resistance. Tomato leaves treated with appropriate concentration of each synergist were fed to second instar larvae for 12 h. The concentrations of PBO, DEF, and DEM were 100, 100 and 120 mgL-1, respectively. These were the highest concentrations that caused no mortality in susceptible strain in preliminary tests. The cytochrome P450-dependent monooxygenase- inhibitor PBO significantly
synergized the activity of deltamethrin in the three greenhouse field populations (with the highest
synergism ratio of 5.4). No significant synergism of deltamethrin toxicity was observed when larvae were pretreated with the esterase-inhibitor DEF, and the glutathione depleter DEM, as indicated by the overlap in the 95% CL for treatment with deltamethrin alone or with deltamethrin +DEF/ DEM. 

کلیدواژه‌ها [English]

  • deltamethrin
  • field population
  • resistant to pesticides
  • Tomato leaf miner
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