شناسایی مولکولی و ریخت‌شناسی دو گونه از کنه‌های خانواده‌Pachylaelapidae Berlese (Acari: Mesostigmata) در استان گیلان

نوع مقاله : مقاله کامل، فارسی

نویسندگان

1 گروه گیاهپزشکی ، داتشکده ی علوم کشاورزی، دانشگاه گیلان، رشت ، ایران

2 گروه گیاهپزشکی، دانشکده ی علوم کشاورزی، دانشگاه گیلان، رشت، ایران

3 گروه گیاه‌پزشکی، دانشکده‌ی علوم کشاورزی، دانشگاه گیلان، رشت، ایران

4 گروه حشره‌شناسی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

چکیده

کنه­های خانواده­Berlese, 1913  Pachylaelapidae کنه­هایی شکارگر با زندگی آزاد هستند و از بندپایان و نماتودهای خاک­زی تغذیه می­کنند. از آن­جا که تعداد قابل توجهی از کنه­های این خانواده شباهت­های ریخت­شناسی به هم داشته و همچنین توصیف­کنندگان تاکسون­های جدید به برخی از ویژگی­های ریخت­شناسی استناد کرده­اند که وجه تمایز کافی برای افتراق آن­ها از تاکسون­های نزدیک را ندارند، شناسایی برخی از این کنه­ها دشوار است. در این موارد استفاده از روش­های نوین شناسایی می­تواند در این زمینه راه­گشا باشد. بنابراین، در این بررسی تلاش شده تا همراه با شناسایی بر مبنای ویژگی­های ریخت­شناسی و اندازه­ای (شناسایی مورفومتریک)، از روش مولکولی بر پایه­ی توالی­یابی ناحیه­ ژنی ITS
(Internal Transcribed Spacer) برای شناسایی دو گونه­ Olopachys compositus Koroleva, 1976 و Olopachys caucasicus Koroleva, 1976 استفاده شود. به این منظور پس از استخراج DNA، با استفاده از آغازگر مناسب ناحیه­ی ITS این دو گونه تکثیر و توالی­یابی شدند و توالی­های به دست آمده در بانک ژن ثبت شد. پس از هم­ردیف­سازی توالی­ها، میزان اختلاف ژنتیکی بین آن­ها 3/2 درصدمحاسبه شد. در نتیجه­ این بررسی­ها، علاوه بر تفکیک دو گونه­ O. compositus و O. caucasicus بر اساس اختلافات ریختی، روش مولکولی نیز به خوبی اختلاف این دو گونه را تأیید کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Molecular and morphological identification of two species of mites of the family Pachylaelapidae Berlese (Acari: Mesostigmata) in Guilan province

نویسندگان [English]

  • R. Hosseini 1
  • Samaneh Mojahed 2
  • J. Hajizadeh 3
  • Ali Ahadiyat 4
1 Department of Plant protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht
3 Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
4 Department of Agricultural Entomology, Faculty of Agriculture And Natural Resources, Islamic Azad University, Branch of Sciences and Researches, Tehran, Iran
چکیده [English]

Mites of the family Pachylaelapidae Berlese, 1913 are a group of predatory mites with free-living species, feeding on arthropods and soil-inhabiting nematodes. Identification of these mites is difficult, because of morphological similarities in a significant number of species and also the fact that new taxons descriptors have referred to some morphological features that have not sufficient distinction criteria to differentiate them from close taxons. Therefore, the use of new identification methods can be helpful in this context. In the present study, along with identification, based on morphological characteristics and size (morphometric identification), we used a molecular method, based on the sequencing of the internal transcribed spacer (ITS) gene to identify  two species Olopachys compositus Koroleva, 1976 and Olopachys caucasicus Koroleva, 1976. After DNA extraction, ITS region were amplified and sequenced, using the appropriate primers and afterward the sequences were recorded in GeneBank. Alignment of sequences was performed and the genetic differences between species was calculated as 2.3 percent. As a result of these investigations, in addition to the separation of the two species O. compositus and O. caucasicus based on morphological differences, the molecular method also well confirmed the difference between these two species.

کلیدواژه‌ها [English]

  • Mite
  • ITS
  • Sequencing
  • Olopachys
  • Pachylaelapidae

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