بررسی اثر کنه‌کشی روغن و عصاره متانولی بذر منداب Eruca sativa روی (Tetranychus urticae (Red form)(Acari:Tetranichidae

نوع مقاله : مقاله کامل، فارسی

نویسندگان

1 دانشگاه تربیت مدرس، دانشکده کشاورزی، گروه حشره شناسی کشاورزی، تهران، ایران

2 دانشگاه تربیت مدرس، دانشکده کشاورزی، گروه علوم باغبانی، تهران، ایران

چکیده

در این پژوهش اثر کنه‌‌کشی روغن و عصاره‌ی متانولی بذر منداب Eruca sativa (Miller) (Brassicaceae) روی کنه Tetranychus urticae (Red form)(Acari:Tetranychidae)در شرایط آزمایشگاهی مورد ارزیابی قرار گرفت. تلفات کنه‌های بالغ بعد از 24 ساعت ارزیابی و مقادیر LC50 روغن و عصاره محاسبه شد. این مقادیر برای روغن و عصاره‌ی متانولی بذر منداب به ترتیب 3062 و 5674 میلی‌گرم بر لیتر به دست آمد. اندازه‌گیری میزان ایزوتیوسیانات روغن و عصاره متانولی بذر منداب با روش کروماتوگرافی مایع با کارآیی بالا (HPLC) نشان داد که میزان ایزوتیوسیانات اروسین در روغن منداب نسبت به عصاره متانولی آن بالاتر می‌باشد. همچنین آنالیز اسیدهای چرب روغن بذر منداب با استفاده از کروماتوگرافی گازی (GC) وجود 12 نوع اسید چرب را در روغن نشان داد که سطح اسید‌های چرب آراشیدونیک، اولئیک و گاما‌لینولنیک در آن بالا بود. با توجه به پایین بودن میزان ایزوتیوسیانات‌ اروسین و اسیدهای چرب در عصاره متانولی بذر منداب می­توان علت کنه‌‌کشی بیشتر روغن منداب را به نوع و مقدار ایزوتیوسیانات و اسیدهای چرب آن نسبت داد. بنابراین یافته‌های ما نشان داد که حضور فیتوکمیکال‌های فعال در روغن E. sativa نقش مهمی در اثر بخشی آن علیه کنه T. urticae  دارد.

کلیدواژه‌ها

عنوان مقاله [English]

Acaricidal activity of Eruca sativa seed oil and its methanolic extract on Tetranychus urticae (Red form)(Acari:Tetranichidae)

نویسندگان [English]

  • S. Moharramipour 1
  • M. Masoumi 1
  • Mehdi Ayyari 2
1 Department of Agricultural Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2 Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
چکیده [English]

In this study, contact toxicity of Eruca sativa (Miller) (Brassicaceae) oil and its methanolic extract were examined on the adult female of Tetranychus urticae (Red form)) (Tetranychidae) at five concentrations and three replications set at 25 ± 1°C, 65 ± 5% RH and 16: 8 h (light: dark). The mortality was evaluated 24 h after treatment. The LC50s of E. sativa oil and the methanol extract were 3062 and 5674 mg L-1, respectively. Measurement of erucin as the main isothiocyanate compound in the oil and methanolic seed extract using high performance liquid chromatography (HPLC) showed that the amount of erucin in the oil was higher than methanol extract. Also, analysis of fatty acids in the oil using gas chromatography (GC) showed that there were 12 types of fatty acids in oil, high level of arachidonic, oleic and Ƴ-linolenic acids. Due to the biological activity and phytochemical analysis, it can be concluded that the higher amount of erucin and fatty acids in the oil, caused the higher acaricidal activity of E. sativa oil compared to methanolic extract. Therefore, our findings showed that the presence of main active phytochemicals in the E. sativa oil plays a decisive role in its efficacy.

کلیدواژه‌ها [English]

  • Eruca sativa oil
  • Isothiocyanate
  • fatty acid
  • acaricid

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