اثر پروتکسین بر قابلیت هضم نشاسته ذرت توسط زنبور عسل، Apis melifera (Hymenoptera: Apidae)

نوع مقاله : مقاله کامل، انگلیسی

نویسندگان

1 گروه علوم دامی دانشکده علوم کشاورزی و صنایع غذایی دانشگاه آزاد اسلامی واحد علوم تحقیقات، تهران، ایران

2 گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

این پژوهش به منظور بررسی توان زنبور عسل در مصرف نشاسته به عنوان جایگزین شهد و اثر پروبیوتیک تجاری پروتکسین بر قابلیت هضم نشاسته در دستگاه گوارش زنبور عسل انجام شد. در آزمایش اول تعداد 36 کندو به طور تصادفی در شش گروه همسان قرار گرفتند و هر گروه یکی از جیره­های آزمایشی، تیمار اول جیره فقط بر پایه شربت شکر (گروه شاهد)، تیمار دوم جیره پایه حاوی یک گرم بر لیتر پروتکسین، گروه سوم جیره پایه حاوی 10 درصد نشاسته، گروه چهارم جیره پایه حاوی 20 درصد نشاسته (S20)، گروه پنجم جیره پایه حاوی 10 درصد نشاسته و یک گرم بر لیتر پروتکسین (S10P) و گروه ششم جیره پایه حاوی 20 درصد نشاسته و یک گرم بر لیتر پروتکسین (S20P) را دریافت کردند. در آزمایش دوم زنبورهای تازه ظاهر شده در شش گروه در داخل قفس­هایی در انکوباتور قرار داده شده و تیمارهای اشاره شدة بالا را به مدت 21 روز دریافت کردند (در دمای 1 ± 34 درجه سلسیوس و رطوبت نسبی 50 درصد). در پایان هر دو آزمایش تعداد 100 عدد زنبور کارگر از هر تیمار انتخاب و از نظر میزان جذب نشاسته، وزن خشک، جمعیت میکروبی بخش­های مختلف دستگاه گوارش، غلظت پروتئین و چربی بدن آن­ها ارزیابی شدند. نتایج این آزمایش نشان داد، پروتکسین به طور معنی­داری جذب نشاسته را در گروه S20P نسبت به گروه­های دیگر افزایش داد (P≤0.05). همچنین حضور نشاسته در جیره زنبورها سبب افزایش معنی­دار وزن خشک، غلظت پروتئین و چربی بدن زنبورها شد (P≤0.05). پروتکسین جمعیت میکروبی را در بخش­های مختلف دستگاه گوارش زنبورها افزایش داد (P≤0.05). بر اساس نتایج این آزمایش به نظر می­رسد افزودن همزمان نشاسته و پروتکسین می­تواند تاثیر مثبتی بر سلامتی و قدرت کلنی داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Impact of Protexin on digestibility of corn starch by honey bee Apis mellifera (Hymenoptera: Apidae)

نویسندگان [English]

  • M. Hasan Vakili 1
  • M. Chamani 1
  • Gh. A. Nehzati 2
  • A. A. Sadeghi 1
1 Department of agriculture and food science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of animal science, College of agriculture and natural resources, University of Tehran, Karaj, Iran
چکیده [English]

This study was conducted to investigate the ability of bees to consume starch as a substitute for nectar and influence a commercial probiotic namely Protexin® on starch absorption. In the first experiment, 36 honey bee hives were randomly allocated into six similar groups and fed using one of the diets, first group received sugar syrup as control treatment, second group received sugar syrup supplemented with 1 g/L of Protexin® (P), third group received sugar syrup supplemented with 10% of the starch (S10), fourth group fed using sugar syrup supplemented with 20% of the starch (S20), fifth group received sugar syrup supplemented with 10% starch and one g/L of Protexin® (S10P) and sixth group fed using sugar syrup supplemented with 20% of starch and one g/L of Protexin® (S20P). In the second experiment, newly emerged worker bees were kept in laboratory cages and fed using the above-mentioned experimental treatments for 21 days (at 34 ± 1 ˚C and 50% R.H.). At the end of both experiments, 100 worker bees from each treatment were selected to evaluate the starch absorption, the microbial population at the bee’s digestive tract, body weight, body protein, and lipid content. The results indicated that the starch absorption in the colonies fed by S20P treatment was significantly higher than that in the rest of the treated colonies (P≤0.05). The supplementation of diet with starch significantly enhanced their body weight, protein, and lipid content in both of the experiments (P≤0.05). Moreover, Protexin® increased the bee’s gut microbial population at colony and cage conditions (P≤0.05). It is concluded that the dietary supplementation of the corn starch and Protexin® could have a beneficial effect on the health and strength of the bee colonies.

کلیدواژه‌ها [English]

  • Microbial population
  • Probiotic
  • Starch absorption
  • Worker bees

مراجع

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