اطلاعاتی نوین درباره نماتودهای حشرات در ایران: گزارش جنس Distolaberellus و نر گونه Oscheius tipulae

نوع مقاله : مقاله کامل، انگلیسی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 گروه بیوشیمی، میکروبیولوژی و بیوتکنولوژی، دانشگاه لیمپوپو، سوونگا، آفریقای جنوبی

3 گروه گیاهپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران

10.61186/jesi.45.1.3

چکیده

 به منظور شناسایی نماتودهای مرتبط با حشرات در مناطق جنگلی استان گیلان، ایران، نمونه‌ های خاک، چوب و حشره جمع‌ آوری شد. نماتودها با استفاده از روش طعمه‌ گذاری حشرات استخراج شده و نمونه‌ های به ‌دست‌ آمده بر اساس ویژگی‌ های مورفولوژیکی و مورفومتری تحلیل شدند. این بررسی به کشف جمعیت‌ هایی متعلق به جنس‌های Distolabrellus  و Oscheius  منجر شد که به ترتیب به عنوان D. veechi و O. tipulae شناسایی شدند. شناسایی مولکولی از طریق تحلیل مولکولی شامل ناحیه D2-D3 ژن 28S، ITS و 18S ژن‌های rRNA انجام شد. تحلیل‌های فیلوژنتیکی این نماتودها را با پشتیبانی بالا در کلادهای مربوطه‌ شان قرار داده و هویت آنها را تأیید کرد. این بررسی برای اولین بار منجر به شناسایی D. veechi و نر O. tipulae در ایران شد و آنها را به عنوان رکوردهای جدید برای کشور معرفی کرد.

چکیده تصویری

اطلاعاتی نوین درباره نماتودهای حشرات در ایران: گزارش جنس Distolaberellus و نر گونه Oscheius tipulae

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

New information on insect nematodes from Iran: report of Distolabrellus veechi (Nematoda: Mesorhabditidae) as new species and male of Oscheius tipulae (Nematoda: Rhabditidae)

نویسندگان [English]

  • Parissa Jalalinasab 1
  • Ramin Heydari 1
  • Reza Talaei-Hassanloui 1
  • Ebrahim Shokoohi 2
  • Javad Karimi 3
1 Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Department of Biochemistry, Microbiology, and Biotechnology, University of Limpopo, Sovenga, South Africa
3 Department of Plant Protection, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

In order to identify nematodes associated with insects in the forested areas of Guilan province, Iran, soil, wood, and insect samples were collected. Nematodes were extracted using the insect-baiting method, and the obtained specimens were analyzed based on both morphological and morphometric characteristics. This investigation led to the discovery populations belonging to the genera Distolabrellus and Oscheius, identified as D. veechi and O. tipulae, respectively. Molecular identification was carried out through molecular analysis, encompassing the D2-D3 expansion segment of 28S, ITS, and 18S genes of rRNA. The phylogenetic analyses placed these nematodes within their respective clades with high bootstrap support, confirming their identity. This survey marks the first discovery of D. veechi and male O. tipulae in Iran, introducing them as new records for the country.

کلیدواژه‌ها [English]

  • Insect nematology
  • Insect pathology
  • Biocontrol
  • Molecular Charzterization
  • Phylogeny

© 2025 by Author(s), Published by the Entomological Society of Iran

This Work is Licensed under Creative Commons Attribution-Non Commercial 4.0 International Public License

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