بررسی تاثیر تنش‌های دمایی روی سامانه ایمنی بید سیب‌زمینی Phthorimaea operculella (Lepidoptera: Gelechiidae)

نوع مقاله : دومین کنگره بین‌المللی حشره شناسی

نویسندگان

1 دانشجوی دانشگاه صنعتی شاهرود

2 عضو هیات علمی دانشگاه صنعتی شاهرود

چکیده

سلول‌های خونی حشرات، یکی از اجزای مهم سیستم ایمنی حشرات در برابر انواع تنش­ها مانند حمله بیمار‌گرها، پارازیتوئید‌ها، دوره گرسنگی و تغییرات دما می‌باشند. شناخت ویژگی‌های سلول‌های خونی و فراوانی آن‌ها در مطالعات ایمنی‌شناسی سلولی در جهت کنترل بهتر آفت به ما کمک خواهد کرد. در این تحقیق سلول‌های خونی لارو سن چهار Phthorimaea operculella (Zeller) پس از رنگ‌آمیزی با Giemsa با میکروسکوپ نوری و بزرگ‌نمایی 40 شناسایی شدند. 5 نوع هموسیت در حشره بید سیب‌زمینی شناسایی شد که شامل پروهموسیت‌ها، پلاسموتوسیت‌ها، گرانولوسیت‌ها، اونوسیتوئید‌ها و اسفرولوسیت‌ها بودند. اثر تنش­های دمایی مختلف نیز به مدت 24 ساعت بر دفاع سلولی لارو‌های سن چهارم بید سیب‌زمینی بررسی شد. به علاوه تعداد هموسیت­های مختلف و تعداد کل سلول­های خونی بررسی شدند. نتایج نشان داد که تعداد کل هموسیت‌ها و پلاسموتوسیت‌های لارو‌هایی که به مدت 24 ساعت تحت تنش دمایی 35 درجه سانتی‌گراد قرار گرفتند، نسبت به شاهد (دمای 1±25 درجه سانتی گراد) به طور معنی­داری افزایش یافت. همچنین در اثر تنش سرما (دمای 4 درجه سانتی گراد)، تعداد کل هموسیت‌ها، پلاسموتوسیت‌ها و اونوسیتوئید‌ها نسبت به شاهد کاهش معنی‌داری نشان داد. این یافته­ها می­تواند به عنوان مقدمه‌ای برای تحقیقات بیشتر در راستای مطالعات ایمنی­شناسی بید سیب‌زمینی مورد استفاده قرار گیرد.
 

کلیدواژه‌ها

عنوان مقاله [English]

The effect of thermal stresses on the immune system of the potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae)

نویسندگان [English]

  • z. Pourali 1
  • Maryam Ajam Hassani 2
1 student in Shahrood University of Technology
چکیده [English]

The hemocytes of insects are one of important components of immune system of insects against various stresses such as pathogens attack, parasitoids, starvation period and temperature changes. Hemocytes characteristics recognition and frequency in cellular immune studies will help us in order to better pest control. In this study hemocytes of fourth instar larvae of potato tuber moth Phthorimaea operculella (Zeller)were identified after staining with Giemsa and by light microscopy at 40x magnification. Five types of identified hemocytes were prohemocytes (PRs), plasmatocytes (PLs), granulocytes (GRs), oenocytoids (OEs) and spherulocytes (SPs). The effect of different thermal stresses was also investigated for 24 hours on cellular defense of fourth instar larvae. In addition number of various hemocytes and total number of blood cells were investigated. At 35 °C, total hemocyte count (THC) and PLs of larvae was increased significantly compared to the control (25±1 °C). Also, chill stress
(4 °C) showed a significant decrease in THC, PLs and OEs compared to the control. These findings could be used as a base for further investigation on the immunology studies of potato tuber moth.

 

کلیدواژه‌ها [English]

  • Phthorimaea operculella
  • Hemocytes
  • Cellular defense
  • heat and chill stresses
  • Total Hemocyte Count

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