مطالعه انگل شناسی و ملکولی نوزموزیس در زنبورستان های مهاجر در استان هرمزگان ،جنوب ایران

نوع مقاله : مقاله کامل، انگلیسی

نویسندگان

1 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد ، ایران

2 اداره کل دامپزشکی استان هرمزگان، بندر عباس، ایران

10.61186/jesi.43.4.6

چکیده

نوزموزیس یک بیماری میکروسپوریدیایی ناشی از Nosema ceranae و Nosema apis است که باعث آلودگی زنبوران بالغ در کلنی­ های زنبورعسل در سراسر جهان می­ گردد. انتقال اسپورهای نوزما از طریق مسیرهای مدفوعی-دهانی و دهانی– دهانی اتفاق می ­افتد. با توجه به اهمیت سلامت زنبورهای عسل، هدف از این مطالعه بررسی فراوانی آلودگی با نوزما در زنبوداری های مهاجر به استان هرمزگان با استفاده از روش‌های میکروسکوپی و مولکولی بود. بدین منظور از 84 زنبورستان مهاجر در استان هرمزگان نمونه ­برداری شد، بدین صورت که از هر زنبورستان 10 کندو انتخاب شده و از هر کندو 20 تا 30 زنبورعسل مسن به صورت تصادفی انتخاب شده و به آزمایشگاه منتقل شدند. در آزمایشگاه، شکم زنبورها با پنس و قیچی حشره ­شناسی از بقیه بدن جدا شده و در هاون حاوی سرم نمکی آسیاب شد. سوسپانسیون تهیه شده با عبور از الک صاف شده و به لوله ­های آزمایش منتقل و سانتریفیوژ گردید. رسوب ته لوله­ های ازمایش به طور مکرر با افزودن سرم فیزیولوژی و سانتریفیوژ شسته شده و برای تشخیص عفونت نوزما با روش­های میکروسکوپی و مولکولی مورد بررسی قرار گرفتند. در بررسی میکروسکوپی، اسپورهای گونه ­هایNosema  در 38.2 درصد زنبورستان ها مشاهده شدند. با روش PCR اما 39.2 درصد زنبورستان ها آلوده به  N. ceranae تشخیص داده شدند. هیچ موردی از آلودگی N. apis مشاهده نشد. با توجه به فراوانی قابل ملاحظه ­ی آلودگی نوزما در زنبورستان های مهاجر به استان هرمزگان، انجام اقدامات بهداشتی مناسب همچون غربال گری زنبوستان های کشوز با روش­های تشخیصی مناسب و آموزش زنبورداران جهت ضدعفونی صحیح کندوها برای کنترل بیماری در زنبورستان های ایران توسط مسئولان بهداشتی دامپزشکی ضروری است.

چکیده تصویری

مطالعه انگل شناسی و ملکولی نوزموزیس در زنبورستان های مهاجر در استان هرمزگان ،جنوب ایران

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Parasitological and molecular study of nosemosis in migratory apiaries in Hormozgan Province, southern Iran

نویسندگان [English]

  • Bahareh Meftahi 1
  • Saeed Yaghfoori 2
  • Sadegh Mosazadeh 2
  • Reza Sheibanitezerji 2
  • Mostafa Fakhrabadipour 2
  • Esmaeil Javdan 2
  • Gholamreza Razmi 1
1 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2 Hormozgan Veterinary Head Office, Bandar Abbas, Iran
چکیده [English]

Nosemosis is a microsporidian disease caused by Nosema ceranae and N. apis and transmitted via oral-fecal and oral-oral routes. It is globally distributed among adult bees in honeybee colonies. Considering the health importance of nosemosis in honeybees, the study aims to determine the frequency of Nosema spp. infection in migratory apiaries in Hormozgan province by microscopic and molecular methods. In the present study, 20 bees from ten randomly selected hives in 84 migratory apiaries were collected. In the laboratory, the abdomen of the bees was separated from the rest of the body with entomological tweezers and scissors and then ground up in a mortar containing saline serum. The prepared suspension was filtered by passing through a sieve, then the prepared suspension was transferred to test tubes and centrifuged. The pellets were repeatedly washed by saline solution and centrifuged. Finally, the pellets were examined for spores of Nosema spp. by light microscopy and conventional PCR. In microscopy, 38.2 % of apiaries were positive for Nosema spp. spores. By PCR however, DNA of N. ceranae was detected in 39.2. % of apiaries with no samples positive for N. apis. Due to the considerable frequency of infection in migratory apiaries in Hormozgan province, it is necessary to carry out appropriate health measures such as screening of apiaries with appropriate diagnostic methods and training of beekeepers to disinfect hives in order to control Nosema infection in Iranian apiaries by the veterinary health officials.

کلیدواژه‌ها [English]

  • Insect pathology, honey bee
  • Iran
  • characterization

© 2023 by Author(s), Published by the Entomological Society of Iran

This Work is Licensed under Creative Commons Attribution-Non Commercial 4.0 International Public License

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نامه انجمن حشره شناسی ایران
دوره 43، شماره 4
آذر 1402
صفحه 383-392

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