The possibility of using artificial oviposition substrates for mass rearing of Sesamia cretica and S. nonagrioides (Lep.: Noctuidae) under laboratory condition

Document Type : Paper, Persian

Authors

1 Plant Protection Research Department, Khuzestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran

2 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Successful mass rearing of Telenomus busseolae Gahan relies on the large-scale production of Sesamia spp. eggs, in Iran. The present study aimed to investigate the effect of artificial substrates (filter paper, parafilm, wax paper, and cellophane) in both dry and wet form, on the oviposition of S. cretica Lederer and S. nonagrioides Lefebvre as the main treatments, with and without an oviposition stimulant compared to sugarcane stem (shoot) as the control treatment, across 20 replications in a completely randomized design. Each artificial substrate was soaked with 1 ml. of corn leaf extract (oviposition stimulant) and placed in a container of water. All experiments were conducted at 27±1°C, 50±10% RH, and a photoperiod of 16:8 h (L:D). Based on the results obtained, the highest egg-laying for S. cretica and S. nonagrioides were observed in natural host (sugarcane shoot) with 156.92± 5.934 and 324.89± 15.274 eggs per female, respectively. Two artificial substrates, moist cellophane and parafilm (with the oviposition stimulant), were grouped together in terms of fecundity, following the natural host treatment with 71.3± 2.019 and 64.95±1.609 eggs per female in S. cretica and 142.6± 3.260 and 136.65± 3.546 eggs per female in S. nonagrioides, respectively. The cellophane substrate, due to its lower adhesion, easier application, and lower cost compared to parafilm, is more efficient egg-laying substrate for moths. Overall, despite the problems of using natural substrates, for the mass rearing of Sesamia spp. in insectarium, the use of the sugarcane shoots during mating and egg-laying stages is superior to artificial substrates. However, if access to natural host shoots, was not possible, especially in laboratory studies, a moist cellophane substrate with corn leaf extract would serve as a suitable alternative. The results of this research can facilitate the mass rearing of Sesamia spp. under laboratory condition and in insectariums.

Graphical Abstract

The possibility of using artificial oviposition substrates for mass rearing of Sesamia cretica and S. nonagrioides (Lep.: Noctuidae) under laboratory condition

Keywords

Main Subjects

Article Title [Persian]

امکان به‌کارگیری بسترهای مصنوعی تخم‌ریزی برای پرورش انبوه ساقه‌خوارهای Sesami cretica وS. nonagrioides (Lep.: Noctuidae) در شرایط آزمایشگاهی

Authors [Persian]

  • افروز فارسی 1
  • حسین رنجبر اقدم 2
1 بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان خوزستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران
2 مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
Abstract [Persian]

در ایران، پرورش انبوه موفق زنبور Telenomus busseolae Gahan، به تولید انبوه تخم‌ گونه‌های Sesamia spp.، وابسته است. پژوهش حاضر با هدف بررسی امکان جایگزینی بسترهای مصنوعی (کاغذ صافی، پارافیلم، کاغذ روغنی و سلفون) به دو صورت خشک و مرطوب برای تخم‌ریزی گونه‌های S. cretica Lederer و S. nonagrioides Lefebvre به عنوان تیمارهای اصلی به‌ترتیب همراه با محرک تخم‌ریزی و بدون آن در مقایسه با ساقه نیشکر (شوت) به عنوان تیمار شاهد در 20 تکرار و در قالب طرح کاملاً تصادفی انجام شد. هر بستر مصنوعی با یک میلی‌لیتر عصارة برگ ذرت (محرک تخم‌ریزی) آغشته و در ظرف حاوی آب قرار داده شد. تمامی آزمایش‌ها در دمای 1±27 درجة سلسیوس، رطوبت نسبی10±50 درصد و دورة نوری 8: 16 ساعت (تاریکی: روشنایی) انجام شد. بر اساس نتایج به‌دست آمده، بیشترین تخم‌ریزی گونه‌هایS. cretica و S. nonagrioides، به‌ترتیب به‌ میزان 934/5±92/156و 274/15±89/324 تخم به ازای هر ماده روی شوت نیشکر مشاهده شد. دو بستر سلفون و پارافیلم مرطوب همراه با عصارة برگ ذرت به‌ترتیب به میزان 019/2±3/71 و 609/1±95/64 تخم به ازای هر ماده در گونةS. cretica و 260/3±6/142 و 546/3±65/136 تخم به ازای هر ماده در گونة S. nonagrioides، در یک گروه و بعد از میزبان طبیعی قرار گرفتند. از سوی دیگر، بستر سلفون به-دلیل چسبندگی کمتر، کاربرد آسان‌تر و هزینه کمتر نسبت به پارافیلم کارایی بیشتری برای تأمین بستر تخم‌ریزی شب‌پره-ها داشت. در مجموع، علی‌رغم مشکلات به کارگیری بسترهای طبیعی، به ‌منظور پرورش انبوه شب‌پره‌های Sesamia spp. در اینسکتاریوم، استفاده از شوت‌های نیشکر در مرحله تلاقی و تخم‌ریزی نسبت به بسترهای مصنوعی برتری دارد. ولی اگر دسترسی به شوت‌های میزبان طبیعی امکان‌پذیر نبود، به‌ویژه در مطالعات آزمایشگاهی، استفاده از بستر سلفون مرطوب همراه با عصارة برگ ذرت مناسب خواهد بود. نتایج این تحقیق می‌تواند در راستای تسهیل پرورش‌های انبوه ساقه‌خواران Sesamia spp. در شرایط آزمایشگاهی و اینسکتاریوم‌ها مؤثر باشد.

Keywords [Persian]

  • کنترل بیولوژیک
  • مدیریت آفات
  • اینسکتاریوم
  • شوت نیشکر
  • عصارة برگ ذرت

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Journal of Entomological Society of Iran
Volume 45, Issue 3
July 2025
Pages 465-477

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