DNA barcoding of Indian species of genus Lucilia (Dip., Calliphoridae) with two new country records

Document Type : Paper, English

Authors

1 Department of Zoology and Environmental Sciences, Punjabi University, Patiala, India

2 Department of Zoology, University of Kashmir, Srinagar, Jammu & Kashmir, India

10.22034/jesi.46.1.4

Abstract

Lucilia is a genus of flies causing myiasis and breeding in carrion making them a group of medical, veterinary and forensic importance. From India, the genus is represented by 8 species so far. Herein, we have reported two new records for the country (Lucilia bismarckensis Kurahashi and Lucilia silvarum (Meigen)) and provided an identification key to the known Indian species of the genus Lucilia.  Cytochrome oxidase subunit 1 (COI) sequences for three Indian species were generated and analyzed along with seven publicly available sequences retrieved from the GenBank, using Maximum Likelihood analysis. We found that COI could be used as a reliable marker for DNA based identification of the group as the species in question showed enough interspecific divergence and formed well supported clades.

Graphical Abstract

DNA barcoding of Indian species of genus Lucilia (Dip., Calliphoridae) with two new country records

Keywords

Main Subjects

Article Title [Persian]

دی ان ای بارکدینگ گونه‌های هندی جنس (Dip., Calliphoridae) Lucilia به همراه دو رکورد جدید کشوری

Authors [Persian]

  • میناکشی بهاتری 1
  • تنویر احمد دار 2
1 گروه جانورشناسی و علوم محیطی، دانشگاه پنجاب، پاتیالا، هند
2 گروه جانورشناسی، دانشگاه کشمیر، سرینگر، جامو و کشمیر، هند
Abstract [Persian]

Lucilia جنسی از مگس‌ها است که باعث ایجاد میاز و تولید مثل در لاشه حیوانات می‌شود و همین امر آنها را به گروهی با اهمیت در دامپزشکی و پزشکی قانونی تبدیل کرده است. این جنس تاکنون در هند با ۸ گونه شناخته شده است. در اینجا، دو رکورد جدید شامل گونه های Lucilia bismarckensis Kurahashi و Lucilia silvarum Meigen برای هندوستان گزارش می‌شود و یک کلید شناسایی برای گونه‌های شناخته شده هندی جنس Lucilia ارائه گردیده است. توالی‌های زیر واحد ۱ سیتوکروم اکسیداز (COI) برای سه گونه تعیین گردید و این توالی ها به همراه هفت توالی متناظر این ناحیه ژنی که از بانک ژن اخذ شده بود با استفاده از آنالیز حداکثر احتمال، تجزیه و تحلیل شدند. این بررسی نشان داد که COI می‌تواند به عنوان یک نشانگر قابل اعتماد برای شناسایی مولکولی این گروه استفاده شود، زیرا گونه‌های مورد نظر، واگرایی بین گونه‌ای کافی را نشان دادند و کلادهای قابل اعتمادی تشکیل دادند. 

Keywords [Persian]

  • شناسایی مولکولی
  • حشره‌شناسی قانونی
  • Lucilia bismarckensis
  • Lucilia silvarum

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Journal of Entomological Society of Iran
Volume 46, Issue 1
January 2026
Pages 39-48

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