The effects of Pirimicarb Insecticide on the Physiological Indices of Rapeseed Plant

Document Type : Paper, English

Authors

1 Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

3 Department of Plant Production and Genetics, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran

10.22034/jesi.46.2.4

Abstract

This study investigated the physiological effects of pirimicarb insecticide application on early-stage rapeseed (Brassica napus L. cv. Hyola 401) plants. Field-grown plants at the 2-4 leaf stage received foliar applications of pirimicarb (500 mg L⁻¹) or distilled water (control) and were subsequently maintained under controlled greenhouse conditions. Physiological measurements conducted 72 hours post-treatment revealed significant increases in total phenolic compounds (+37.4%, P=0.008) and chlorophyll b content (+30.4%, P=0.006) in treated plants. No significant changes were observed in hydrogen peroxide (H₂O₂) levels (+21.1%, P=0.358), malondialdehyde (MDA) concentrations (−14.0%, P=0.086), chlorophyll a (−5.1%, P=0.591), total chlorophyll (+0.8%, P=0.924), or carotenoids (−9.3%, P=0.574). Correlation analysis (n=8 replications) demonstrated a strong positive relationship between phenolic compounds and chlorophyll b (r=0.902, P=0.002), suggesting coordinated metabolic regulation. Strong correlations among core photosynthetic pigments indicated maintained functional integrity of the photosynthetic apparatus. These findings demonstrate that early-stage rapeseed plants respond to pirimicarb exposure through specific metabolic adjustments involving phenolic biosynthesis and chlorophyll b accumulation, without evidence of oxidative damage or photosynthetic impairment. The results support continued use of pirimicarb in integrated pest management for rapeseed cultivation.

Graphical Abstract

The effects of Pirimicarb Insecticide on the Physiological Indices of Rapeseed Plant

Keywords

Main Subjects

Article Title [Persian]

اثرات حشره کش پیریمیکارب روی شاخص های فیزیولوژیک گیاه کلزا

Authors [Persian]

  • محمد حیدری 1
  • بهزاد حبیب پور 1
  • هادی مصلی نژاد 2
  • حبیب اله روشنفکر 3
1 گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، ایران
2 موسسه تحقیقات گیاه پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی تهران، ایران
3 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، ایران.
Abstract [Persian]

این مطالعه به بررسی اثرات فیزیولوژیک حشره‌کش پیری‌میکارب بر گیاه کلزا در مراحل اولیه رشد می‌پردازد. گیاهان رشد‌یافته در مزرعه، در مرحله 2-4 برگی، محلول‌پاشی برگی پیری‌میکارب (500 میلی‌گرم بر لیتر) یا آب مقطر (شاهد) دریافت کردند و سپس تحت شرایط کنترل‌شده گلخانه نگهداری شدند. اندازه‌گیری‌های فیزیولوژیک که 72 ساعت پس از تیمار انجام شد، افزایش معناداری را در ترکیبات فنولی کل (۴٪/۳۷، ۰۰۸/۰ P=) و کلروفیلb (۴٪/۳۰، ۰۰۶/۰P=) در گیاهان تیمارشده نشان داد. تغییرات معناداری در سطوح پراکسید هیدروژن (H₂O₂) (۱٪/۲۱+، ۳۵۸/۰ P=). مالون‌دی‌آلدئید (۰٪/۱۴-، ۰۸۶/۰ P=)، کلروفیل a (۱٪/۵-، ۵۹۱/۰ P=)، کلروفیل کل (۸٪/۰+، ۹۲۴/۰ P=)، یا کاروتنوئیدها (٪۳/۹-، ۵۷۴/۰ P=)، مشاهده نشد. تحلیل همبستگی (۸ n=تکرار) یک رابطه مثبت قوی میان ترکیبات فنولی و کلروفیل b (۹۰۲/ r=،۰۰۲/۰ P=) را نشان داد که بیانگر تنظیم متابولیکی هماهنگ است. همبستگی‌های قوی میان رنگیزه‌های فتوسنتزی اصلی، نشان‌دهنده حفظ یکپارچگی عملکردی دستگاه فتوسنتزی بود. این یافته‌ها نشان می‌دهد که گیاهان کلزا در مراحل اولیه رشد به قرارگیری در معرض پیری‌میکارب از طریق تنظیمات متابولیکی خاص شامل بیوسنتز ترکیبات فنولی و تجمع کلروفیل بدون شواهدی از آسیب اکسیداتیو یا اختلال فتوسنتزی b پاسخ می‌دهند. نتایج از ادامه استفاده از پیری‌میکارب در مدیریت تلفیقی آفات در محصول کلزا حمایت می‌کند.

Keywords [Persian]

  • کلزا
  • پیری‌میکارب
  • فیزیولوژی
  • شاخص ها

© 2026 by Author(s), Published by the Entomological Society of Iran

This Work is Licensed under Creative Commons Attribution-Non-Commercial 4.0 International Public License.

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Journal of Entomological Society of Iran
Volume 46, Issue 2
April 2026
Pages 181-192

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