Novel alphabaculovirus isolated from Helicoverpa armigera (Lepidoptera: Noctuidae) in Iran: characterization and pathogenicity

Document Type : Paper, English

Authors

1 Seed and Plant Certification and Registration Research Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

10.22034/jesi.2026.371324.1795

Abstract

A novel alphabaculovirus, originally isolated from Helicoverpa armigera dead larvae, was collected from a tomato field in the North West region of Iran. Preliminary virus infection was revealed by microscopic observation of irregularly shaped polyhedral inclusion bodies, and the virus was subsequently confirmed by molecular and sequence analysis. Results revealed that the collected larvae contained Helicoverpa armigera nucleopolyhedrovirus (HearNPV), which was named HearNPV-IR. Phylogenetic analysis based on partial single and concatenated sequences of late expression factor 8 (lef-8), late expression factor 9 (lef-9), and polyhedrin (polh) genes placed the HearNPV-IR isolate within the HearMNPVs clade, near the Russia isolate HearMNPV 3154. Concentration-mortality bioassays were conducted on 3rd instar larvae of H. armigera, and the mean lethal dose (LC50) of HearMNPV-IR was estimated at 4×105 OBs/ml. The ST50 (median survival time) was 3- and 4-days post-infection (dpi) when the 3rd instar larvae were inoculated by OBs concentration equivalent to 3×109 and 3×108 OBs/ml, respectively. The highest dose resulted in 100% mortality at 5 dpi. The desirable insecticidal properties of HearMNPV-IR support its promising biocontrol agent against cotton bollworm.

Graphical Abstract

Novel alphabaculovirus isolated from Helicoverpa armigera (Lepidoptera: Noctuidae) in Iran: characterization and pathogenicity

Keywords

Main Subjects

Article Title [Persian]

آلفاباکولوویروس جدید از ایران روی Helicoverpa armigera: شناسایی و بیماری زایی

Authors [Persian]

  • راحله شهبازی پاشاکی 1
  • مسعود نادرپور 1
  • رضا طلایی حسنلویی 2
1 موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران
2 گروه گیاهپزشکی، دانشکده‌ی کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران.
Abstract [Persian]

در پژوهش حاضر از لاروهای مرده و بیمار Helicoverpa armigera جمع­ آوری شده از مزارع گوجه فرنگی در مناطق شمال غربی کشور، ویروس چندوجهی هسته ­ای از جنس آلفاباکولوویروس جداسازی و شناسایی گردید. آلودگی به ویروس ابتدا با مشاهده میکروسکوپی اشکال نامنظم و درخشان پلی­ هدرین استخراج شده از مایعات سلولی لارو، مشخص و سپس با آنالیزهای مولکولی و توالی­ یابی، تایید شد. نتایج بررسی­ها گونه Helicoverpa armigera nucleopolyhedrovirus  را در لاروهای جمع­آوری شده اثبات و به اختصار HearNPV-IR نامگذاری شد. مطالعات تبارزایی بر اساس بخشی از توالی­ های منفرد و به ­هم پیوسته ژن­های late expression factor 8 (lef-8)، late expression factor 9 (lef-9) و polyhedrin (polh)  مشخص کرد که جدایه HearNPV-IR در شاخه HearMNPV و در ارتباط نزدیک با جدایه HearMNPV 3154 از روسیه قرار دارد. پاسخ غلظت-مرگ و میر HearMNPV-IR روی لارو سن 3 H. armigera بررسی و متوسط LC50 آن 105 ×4 پلی­ هدر/میلی­ لیتر تخمین زده شد. متوسط زمان بقاء در غلظت­های 109×3 و 108 ×3  پلی­هدر/میلی­ لیتر، به ترتیب 3 و 4 روز پس از بیماریزایی محاسبه گردید. بالاترین دز ویروس، در مدت زمان 5 روز منجر به مرگ و میر 100 درصدی لاروهای آفت شد. نتایج بدست آمده بیانگر ویژگی­های مطلوب حشره­ کشی جدایه HearMNPV-IR به عنوان عامل کنترل بیولوژیک علیه کرم غوزه پنبه است.

Keywords [Persian]

  • بیماری شناسی حشرات
  • کنترل میکروبی
  • شجره شناسی
  • کنترل زیستی

© 2026 by Author(s), Published by the Entomological Society of Iran

This Work is Licensed under Creative Commons Attribution-Non-Commercial 4.0 International Public License.

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