Demographic comparison of Clitostethus arcuatus (Col.: Coccinellidae) feeding on two different whitefly species, Bemisia tabaci and Aleyrodes singularis (Hem.: Aleyrodidae)

Document Type : Paper, English

Authors

1 Biological Control Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

2 Department of Plant Protection, Faculty of Agriculture, Shahed University, Tehran, Iran

3 Insects Taxonomy Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

10.22034/jesi.46.4.2

Abstract

Clitostethus arcuatus (Rossi) is a predatory coccinellid that feeds on whiteflies on various plants, such as ash, pomegranate, mulberry and prickly lettuce. In the present study, biological parameters of C. arcuatus on two whitefly species were studied under laboratory conditions. Two populations of C. arcuatus were separately reared on prickly lettuce leaves (Lactuca serriola L.) infested with Bemisia tabaci (Gennadius) and Aleyrodes singularis Danzig (Hemiptera, Aleyrodidae). Egg cohorts of the predatory ladybird reared on two whitefly species were prepared in plastic Petri dishes. The recorded data on the duration of immature stages and daily oviposition were analyzed and compared using TWOSEX-MSChart software. Results showed that none of the growth parameters of the two ladybird populations were statistically affected. Although, the life expectancy of C. arcuatus reared on the cotton whitefly, B. tabaci, was longer compared with that on A. singularis, the fecundity of the ladybirds fed on A. singularis (287.83 eggs per female) was significantly higher than those fed on B. tabaci (155.51 eggs per female). The highest age-stage reproductive value (vxj) of female C. arcuatus from the A. singularis colony on 34th day (70.26) was more than twice the calculated for ladybird females from the B. tabaci colony on 45th day (31.2). Based on our results and in terms of relative ease of propagation of the host plant, rearing C. arcuatus on prickly lettuce infested with A. singularis has more advantages compared to B. tabaci. 

Graphical Abstract

Demographic comparison of Clitostethus arcuatus (Col.: Coccinellidae) feeding on two different whitefly species, Bemisia tabaci and Aleyrodes singularis (Hem.: Aleyrodidae)

Keywords

Main Subjects

Article Title [Persian]

مقایسه پراسنجه‌های دموگرافی کفشدوزک لکه‌هلالی تغذیه شده با دو گونه سفیدبالک (Hem.: Aleyrodidae)، Bemisia tabaci و Aleyrodes singularis

Authors [Persian]

  • شهرام فرخی 1
  • رضوانه مرادیان 2
  • حبیب عباسی پور 2
  • جلال شیرازی 1
  • شهاب منظری 3
1 بخش تحقیقات کنترل بیولوژیک، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی (تات)، تهران، ایران
2 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شاهد، تهران، ایران
3 بخش تحقیقات رده‌بندی حشرات، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی (تات)، تهران، ایران
Abstract [Persian]

کفشدوزک لکه‌هلالی، Clitostethus arcuatus (Rossi) شکارگر سفیدبالک‌ها روی میزبان‌های گیاهی مختلف از جمله زبان‌گنجشک، انار، توت و کاهو وحشی است. در این بررسی، جدول زندگی و پراسنجه‌های زیستی این کفشدوزک روی دو گونه سفیدبالک در شرایط آزمایشگاه مورد ارزیابی قرار گرفت. به منظور تدارک تخم‌های هم‌زاد از دو جمعیت کفشدوزک‌ شکارگر، کفشدوزک‌های لکه‌هلالی به‌صورت جداگانه روی برگ‌های کاهو وحشی(Lactuca serriola L.)  آلوده به سفیدبالک پنبه Bemisia tabaci (Genn.) و سفیدبالک کاهو وحشی Aleyrodes singularis Danzig داخل ظروف پتری پلاستیکی پرورش داده شدند. داده‌های ثبت شده مربوط به طول دوره‌های رشدی نابالغ و تخم‌ریزی روزانه حشرات ماده با استفاده از نرم‌افزار TWOSEX-MSChart تجزیه آماری و مقایسه شدند. نتایج نشان داد که پراسنجه‌های رشدی دو جمعیت کفشدوزک لکه‌هلالی اختلاف معنی‌داری با یکدیگر نداشتند. با وجود طولانی‌تر بودن امید به زندگی کفشدوزک‌های پرورش یافته روی B. tabaci در قیاس با جمعیت وابسته به A. singularis، میزان باروری کفشدوزک‌های تغذیه شده با A. singularis (83/287 عدد تخم به ازاء هر ماده) به‌صورت معنی‌داری بیشتر از جمعیت پرورش یافته روی سفیدبالک پنبه (51/155 عدد تخم به ازاء هر ماده) بود. بیشترین مقدار ارزش تولیدمثلی سن ـ مرحله (vxj) کفشدوزک‌های لکه‌هلالی جمعیت مربوط به سفیدبالک کاهو وحشی در روز سی و چهارم (26/70) بیش از دو برابر بیشترین میزان محاسبه شده برای کفشدوزک‌های ماده پرورش یافته روی سفیدبالک پنبه در روز چهل و پنجم (2/31) بدست آمد. بر اساس نتایج آزمایش‌های انجام شده و همچنین با توجه به سهولت تکثیر گیاه میزبان، پرورش کفشدوزک لکه‌هلالی روی کاهو وحشی آلوده به سفیدبالک A. singularis، دارای مزیت‌های نسبی بیشتری در مقایسه با سفیدبالک پنبه می‌باشد.

Keywords [Persian]

  • کفشدوزک شکارگر
  • کیفیت طعمه
  • جدول زندگی
  • سفیدبالک
  • کنترل بیولوژیک

© 2026 by Author(s), Published by the Entomological Society of Iran

This Work is Licensed under Creative Commons Attribution-Non-Commercial 4.0 International Public License.

References

Abd-Rabou, S. (2006) Biological control of the pomegranate whitefly, Siphoninus phillyreae (Hom.: Aleyrodidae: Aleyrodinae) by using the bioagent, Clitostethus arcuatus (Col.: Coccinellidae). Journal of Entomology, 3(4), 331-335.
Abd-Rabou, S. & Simmons, A. M. (2010) Augmentation and evaluation of a parasitoid, Encarsia inaron, and a predator, Clitostethus arcuatus, for biological control of the pomegranate whitefly, Siphoninus phillyreae. Archives of Phytopathology Plant Protection, 43(13), 1318-1334. https://doi.org/10.1080/03235400802425762
Agekyan, N. G. (1977) Clitostethus arcuatus Rossi (Coleoptera, Coccinellidae): A predator of citrus whitefly in Adzharia. Entomologicheskoe Obozrenie, 56(1), 31-33.
Akca, E., Ayvaz, T., Yazici, E., Smith, C. & Chi, H, (2015) Demography and population projection of Aphis fabae (Hemiptera: Aphididae): with additional comments on life table research criteria. Journal of Economic Entomology 108(4), 1466-1478. https://doi.org/10.1093/jee/tov187
Atlihan, R. & Chi, H. (2008) Temperature-dependent development and demography of Scymnus subvillosus (Coleoptera: Coccinellidae) reared on Hyalopterus pruni (Homoptera: Aphididae). Journal of Economic Entomology, 101(2), 325-333. https://doi.org/10.1093/jee/101.2.325
Bagheri, S. (2013) Biology and population dynamics of Jasmine whitefly Aleuroclava jasmini (Taka.) (Hem.: Aleyrodidae) and the efficacy of its predator, Clitostethus arcuatus Rossi (Col.: Coccinellidae) on citrus in north of Khuzestan. Dissertation, Shahid Chamran University of Ahvaz.
Bagheri, S., Kocheili, F., Mosaddegh, M. S., Shishebor, P. & Soleymannezhadian, E. (2015) Standardized sampling plan for Jasmine whitefly Aleuroclava jasmini (Takahashi) (Hem.: Aleyrodidae) in Khuzestan’citrus orchards, Southwest of Iran. Plant Pests Research, 4(2), 31-45.
Bellows, T. S., Paine, T., Arakawa, K., Meisenbacher, C., Leddy, P. & Kabashima, J. (1990) Biological control sought for ash whitefly. California Agriculture, 44(1), 4-6.
Bellows, T. S., Paine, Jr. T. D. & Gerling, D. (1992) Development, survival, longevity, and fecundity of Clitostethus arcuatus (Col.: Coccinellidae) on Siphoninus phillyreae (Hom.: Aleyrodidae) in the laboratory. Environmental Entomology, 21(3), 659-663. https://doi.org/10.1093/ee/21.3.659
Chi, H, (1988) Life table analysis incorporating both sexes and variable development rates among individuals. Environmental Entomology, 17, 26-34. https://doi.org/10.1093/ee/17.1.26
Chi, H. (2019) TWOSEX-MSChart: a computer program for the population projection based on age-stage, two sex life table. http://140.120.197.173/Ecology/Download/TWOSEX-MSChart-B100000.rar. Accessed 7 July 2019
Chi, H., Güncan, A., Kavousi, A., Gharakhani, G., Atlihan, R., Özgökçe, M. S., Shirazi, J, Amir-Maafi, M, Maroufpoor, M. & Taghizadeh, R. (2022). TWOSEXMSChart: The key tool for life table research and education. Entomologia Generalis, 42(6), 845–849. https://doi.org/10.1127/entomologia/2022/1851
Chi, H., Kavousi, A., Gharekhani, G., Atlihan, R., Özgökçe, M. S., Güncan, A., Gökçe, A., Smith, C.L., Benelli, G., Guedes, R.N.C., Amir-Maafi, M., Shirazi, J., Taghizadeh, R., Maroufpoor, M., et al. (2023). Advances in theory, data analysis, and application of the age-stage, two-sex life table for demographic research, biological control, and pest management. Entomologia Generalis, 43(4): 705-732. https://doi.org/10.1127/entomologia/2023/2048
Chi, H. & Liu, H. (1985) Two new methods for the study of insect population ecology. Bulletin of the Institute of Zoology, Academia Sinica, 24(2), 225-240.
Evans, G. A. (2008) The whiteflies (Hem.: Aleyrodidae) of the world and their host plants and natural enemies. USDA/Animal Plant Health Inspection Service (APHIS).
Farhadi, R., Allahyari, H. & Chi, H. (2011) Life table and predation capacity of Hippodamia variegata (Coleoptera: Coccinellidae) feeding on Aphis fabae (Hemiptera: Aphididae). Biological Control, 59, 83-89. https://doi.org/10.1016/j.biocontrol.2011.07.013
Farrokhi, S. Ahmadipour, R. Manzari, S. & Yousefi Porshokouh, A. (2025) Predation rate of Clitostethus arcuatus on Aleuroclava jasmini sensu lato, the dominant mulberry whitefly in Tehran green area. Applied Entomology and Phytopathology, 93(1), 97-102.
Farrokhi, S. & Manzari, S. (2021) Study on population fluctuations and identification of mulberry whitefly parasitoids (Hym., Aphelinidae) in Tehran green spaces. Research project final report, Iranian Research Institute of Plant Protection (IRIPP). Accesion No. 59954.
Fisher, R. (1930) The genetical theory of natural selection. Clarendon Press, Oxford, England.
Gerling, D., Alomar, Ò. & Arnò, J. (2001) Biological control of Bemisia tabaci using predators and parasitoids. Crop Protection, 20, 779-799. https://doi.org/10.1016/S0261-2194(01)00111-9
Guershon, M. & Gerling, D. (2001) Parental care in the whitefly Aleyrodes singularis. Ecological Entomology, 26(5), 467-472. https://doi.org/10.1046/j.1365-2311.2001.00345.x
Hodek, I. & Honěk, A. (2009) Scale insects, mealybugs, whiteflies and psyllids (Hemiptera, Sternorrhyncha) as prey of ladybirds. Biological Control, 51(2), 232-243. https://doi.org/10.1016/j.biocontrol.2009.05.018
Kavousi, A., Chi, H., Talebi, K., Bandani, A., Ashouri, A. & Hosseini Naveh, V. (2009) Demographic traits of Tetranychus urticae (Acari: Tetranychidae) on leaf discs and whole leaves. Ecology and Behavior, 102(2), 595-601. https://doi.org/10.1603/029.102.0217
Liotta, G. (1981) Osservazioni bio-etologiche su Clitostethus arcuatus (Rossi) (Col.: Coccinellidae) in Sicilia. Redia, 64, 173-185.
Liotta, G., Agro, A. & Pinto, M. L. (2003). Biological control in citrus groves in the last 50 years: Three successful cases in Western Sicily. Bulletin of IOBC-WPRS, 26(6), 171-173.
Manzari, S. & Farrokhi, S. (2019) Identification and study of the biology of mulberry whitefly, Aleuroclava sp. (Hem.: Aleyrodidae), as well as the identification of its natural enemies in order to control the pest in Tehran green spaces. Research project final report, Iranian Research Institute of Plant Protection (IRIPP). Accesion No. 57378.
Merlin, J., Lemaitre, O. & Gregoire, J. C. (1996) Oviposition in Cryptolaemus montrouzieri stimulated by wax filaments of its prey. Enmmologia Experimentalis et Applicata, 79: 141-146.
Mota, J. A., Soares, A. O. & Garcia, P. V. (2008) Temperature dependence for development of the whitefly predator Clitostethus arcuatus (Rossi). BioControl, 53(4), 603-613.
Nehasil, S. E., Zwolinski, J. P., Dorval, E., & Kurle, C. M. (2026). Intraspecific variation in prey quality affects the consumption rates of top predators. Journal of Animal Ecology, 95, 65–83. https://doi.org/10.1111/1365-2656.70155
Pianka, E. (1994) Evolutionary Ecology. Harper Colins College Publishers, New York.
Pourarian, S., Shirazi, J., Tirgari, S., Shahrokhi Khaneghah, S. & Ahadiyat, A. (2024). Rearing carob moth, Ectomyelois ceratoniae (Zell.), on pistachio nuts: biological and statistical approaches. Phytoparasitica 52(4). https://doi.org/10.1007/s12600-024-01189-9
Reinecke, J. P. (1985) Nutrition: artificial diets. In: Kerkut GA, Gilbert LI, editors. Comprehensive insect physiology, biochemistry and pharmacology. Vol. 4: Regulation: digestion, nutrition, excretion. Oxford: Pergamon Press. Pp 391-420.
Rounagh, H. & Samih, M. A. (2014) The two-sex life table and predation rate of Oenopia conglobata contaminata (Col.: Coccinellidae) feeding on pomegranate green aphid, Aphis punicae (Hem.: Aphididae), under laboratory conditions. Journal of Entomologocal Society of Iran, 34(1), 59-72.
Smucker, M., Allan, J. & Cartertte, B. (2007) A comparison of statistical significance tests for information retrieval evaluation. Paper presented at the 16th ACM conference on information and knowledge management. Lisbon, Portugal.
Southwood, T. & Henderson, A. (2009) Ecological methods. Blackwell Science Ltd. Oxford. UK.
Tavadjoh, Z., Hamzehzarghani, H., Alemansoor, H., Khalghani, J. & Vikram, A. (2010) Biology and feeding behaviour of ladybird, Clitostethus arcuatus, the predator of the ash whitefly, Siphoninus phillyreae, in Fars province, Iran. Journal of Insect Science, 10, Article 120. https://doi.org/10.1673/031.010.12001
Wei, M., Chi, H., Guo, Y., et al. (2020). Demography of Cacopsylla chinensis (Hemiptera: Psyllidae) reared on four cultivars of Pyrus bretschneideri (Rosales: Rosaceae) and P. communis pears with estimations of confidence intervals of specific life table statistics. Journal of Economic Entomology, 113(5), 2343-2353. https://doi.org/10.1093/jee/toaa149
Yang, T. & Chi, H. (2006) Life tables and development of Bemisia argentifolli (Homoptera, Aleyrodidae) at different temperetures. Journal of Economic Entomology, 99(3), 691-698. https://doi.org/10.1093/jee/99.3.691
Yazdani, A. & Assadi, G. H. (1989) Clitostethus arcuatus Rossi (Coleoptera: Coccinellidae) a predator of whitefly and mite on pomegranate trees in Shiraz. In: Abstracts of 9th iranian plant protectioon congress, Mashhad, Iran, 9-14 Sept. 1989.
Yazdani, M. & Samih, M. A. (2012) Life table attributes of Clitostethus arcuatus (Col.: Coccinellidae) feeding on Trialeurodes vaporariorum and Siphoninus phillyreae (Hemiptera: Aleyrodidae). Journal of Asia-pacific Entomology, 15(2), 295-298. https://doi.org/10.1016/j.aspen.2012.01.010
Yazdani, M. & Zarabi, M. (2009) Studies on developmental stages of Clitostethus arcuatus (Coleoptera: Coccinellidae) on ash whitefly with note on a new host, Aleyrodes singularis. Hexapoda, 16(1), 14-17.
Yazdani, M. & Zarabi, M. (2010) Predation of Clitostethus arcuatus (Col., Coccinellidae) on Trialeurodes vaporariorum (Hem., Aleyrodidae). Biocontrol Science and Technology, 20(4), 353-358. https://doi.org/10.1080/09583150903560265
Yazdani, M. & Zarabi, M. (2011) The effect of diet on longevity, fecundity, and the sex ratio of Clitostethus arcuatus (Rossi) (Col.: Coccinellidae). Journal of Asia-pacific Entomology, 14(3), 349-352. https://doi.org/10.1016/j.aspen.2011.04.003
Yu, J. Z., Chi, H. & Chen, B. H. (2005) Life table and predation of Lemnia biplagiata (Coleoptera: Coccinellidae) fed on Aphis gossypii (Homoptera: Aphididae) with a proof on relationship among gross reproduction rate, net reproduction rate and preadult survivorship. Annals of the Entomological Society of America, 98(4), 475-482. https://doi.org/10.1603/0013-8746(2005)098[0475:LTAPOL]2.0.CO;2
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Journal of Entomological Society of Iran
Volume 46, Issue 4
October 2026
Pages 401-414

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