An investigation on fumigant effect of the extract of Origanum vulgare (Lamiales: Lamiaceae) for control of two stored-product beetles

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Abstract

Origanum vulgare L. was collected from Ardebil, Guilan and Mazandaran provinces to assess the fumigant toxicity of its extract and determine LC50 and LC95 values for different developmental stages of Callosobruchus maculatus F. and Tribolium confusum J. du Val. It was extracted by solvents including methanol and dichloromethane. Eggs, larvae and adult of both species were exposed to dichloromethane and methanol extracts of O. vulgare for 72 hours. LC50 and LC95 values of dichloromethane extract were calculated 2084.51 and 4991.24 µl/l air for adults of T. cofusum, and 2282.80 and 6684.71µl/l air for adults of C. maculatus, respectively. Also, LC50 and LC95 values of methanol extract were 1726.57 and 5395.60 µl/l air for adults of T. cofusum, and 2040.88 and 5607.77 µl/l air for adults of C. maculatus, respectively.

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