پاسخ های بویایی زنبور Habrobracon hebetor (Hymenoptera: Braconidae) به ترکیبات شیمیایی فرار کرم گلوگاه انار، Ectomyelois ceratoniae (Lepidoptera: Pyralidae) و گیاه میزبان آن

نوع مقاله : مقاله کامل، فارسی

نویسندگان

1 مؤسسه تحقیقات گیاه پزشکی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی تهران، تهران، ایران

2 گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه تهران، کرج، ایران

چکیده

کیمیاپیام­ها، نقش مهمی در روند انتخاب میزبان توسط انگل­ واره­ ها ایفا می­کنند. در این پژوهش، پاسخ­ های زنبور انگل­ وارة Habrobracon hebetor (Say)  به مواد فرار کرم گلوگاه انار،Ectomyelois ceratoniae Zeller  و میوة انار به عنوان گیاه میزبان آن و مواد  فرار القایی در گیاه میزبان توسط حشره­ گیاه­خوار در بویایی­ سنج دو طرفه مورد بررسی قرار گرفت. نتایج نشان داد، لارو سن آخر کرم گلوگاه انار و فضولات آن، میوة انار دچار آسیب مکانیکی و میوة انار شکافته آلوده به لارو آفت، بیشترین جلب­ کنندگی را برای زنبور انگل ­واره داشتند. مواد فرار کرم گلوگاه انار و فضولات لاروی، با روش ریز ­استخراج بر روی فاز جامد (Solid Phase Micro-Extraction) استخراج شدند. سپس این مواد با بکارگیری گاز کروماتوگرافی طیف سنجی جرمی شناسایی شدند. 40 ترکیب فرار از لارو سن آخر کرم گلوگاه انار و 45 ترکیب فرار از فضولات لاروی شناسایی شدند. ترکیبات اصلی شناسایی شده پنتادکن، نونانال، اکتانال، دکانال، هگزانال و لیمونن بودند. انجام بررسی­ های بیشتر برای ارزیابی جلب­ کنندگی ترکیبات شناسایی شده از E. ceratoniae و همچنین مواد فرار القایی ضروری می­باشد. 

کلیدواژه‌ها

عنوان مقاله [English]

Olfactory responses of Habrobracon hebetor (Hymenoptera: Braconidae) to carob moth, Ectomyelois ceratoniae (Lepidoptera: Pyralidae) and its host plant

نویسندگان [English]

  • Fatemeh Shafaghi 1
  • A. Avand-Faghih 1
  • Seyed Hossein Goldansaz 2
1 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organi-zation (AREEO), Tehran. Iran.
2 Department of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Iran
چکیده [English]

Semiochemicals play a key role on host selection of parasitoids. Responses of parasitoid wasp, Habrobracon hebetor (Say), to volatiles emitted from Ectomyelois ceratoniae Zeller larva, pomegranate as its host plant and herbivorous induced plant volatiles (HIPVs) were investigated using a Y-shaped olfactometer. The results showed that the last larval instar of E. ceratoniae, its feces, the mechanically damaged pomegranates and the infested pomegranates by larva were attractive for the parasitoid wasp. The volatiles of E. ceratoniae larvae and its feces were extracted by solid phase micro-extractionand then identified by GC-MS. Respectively, 40 and 45 components were identified in headspaces of the last larval instar of E. ceratoniae and its feces. The major identified components were1-Pentadecane, Nonanal, Octanal, Decanal, Hexanal and Limonene. More studies are needed to identify HIPVs and also to evaluate the behavioral activity of identified volatiles.

کلیدواژه‌ها [English]

  • Kairomone
  • Synomone
  • Parasitoid
  • Olfactometer
  • Pomegranate
  • Host finding

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