اثر پیری پروکسی فن و دیفنولان بر دگردیسی و فعالیت‌های آنزیمی کرم جوانه خوار تنباکو Spodoptera litura (Fabricius, 1775) (Lepidoptera: Noctuidae)

نوع مقاله : مقاله کامل، انگلیسی

نویسندگان

1 گروه جانورشناسی، دانشگاه الله آباد، پریاگاری، 211002، هند

2 گروه جانورشناسی ، دانشگاه IFTM ، الله آباد ،244102 ، هند

10.61186/jesi.43.4.4

چکیده

کرم جوانه‌خوار تنباکو Spodoptera litura (Lepidoptera: Noctuidae)،آفتی چندخوار روی محصولات کشاورزی است که خسارت اقتصادی شدیدی به بار می‌آورد. در مطالعه حاضر، اثر بیولوژیک و فعالیت آنزیمی دو تنظیم کننده رشد حشرات، پیری پروکسی‌فن و دیفنولان علیه لارو S. litura مورد بررسی قرار گرفت. لاروهای سن ماقبل آخر (0، 24 و 48 ساعته) با دوزهای کشنده (1، 2 و 4 میکروگرم بر میکرولیتر) هر دو آنالوگ هورمونی جوانی (JHAs) به صورت موضعی تیمار شدند. اثرات متعددی از جمله طولانی شدن مدت پوست اندازی لارو به لارو و لارو به شفیره، مرگ و میر لارو و شفیره، خروج ناموفق حشره از مرحله شفیرگی، شکل بینابین، تشکیل تعداد کم شفیره و کاهش ظهور حشره بالغ مشاهده شد. علاوه بر این اثرات در پوست اندازی، هر دو هورمون جوانی دارای اثرات مهاری روی استیل کولین استراز (AChE) بودند اما روی آنزیم گلوتاتیون-S-ترانسفراز (GST) دارای اثرات تحریکی بودند. مقادیر Log IC50 و IC50 پیرپیروکسی فن برای نرخ مهار ظهور بالغین،معادل 98/0- و 1/0 میکروگرم بر میکرولیتر برای لاروهای 24 ساعته، 005/0 و 01/1 میکروگرم بر میکرولیتر برای لاروهای 48 ساعته بود. این مقادیر در دیفنولان برای لاروهای 24 ساعته،  085/0 –و 82/0 میکروگرم بر میکرولیتر بود و در لاروهای 48 ساعته، معادل 09/0 - و 23/1 میکروگرم بر میکرولیتر، محاسبه شد. نتایج نشان داد که هر دو ترکیب تنظیم کننده رشد حشرات، روی لاروهای S. litura دارای قدرت حشره‌کشی هستند.

چکیده تصویری

اثر پیری پروکسی فن و دیفنولان بر دگردیسی و فعالیت‌های آنزیمی کرم جوانه خوار تنباکو Spodoptera litura (Fabricius, 1775) (Lepidoptera: Noctuidae)

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of pyriproxyfen and diofenolan on the metamorphosis and enzymatic activities in tobacco cutworm, Spodoptera litura.

نویسندگان [English]

  • Asmita Basu 1
  • Rahul Maddheshiya 2
1 Department of Zoology, University of Allahabad, Prayagraj (211002), India.
2 Entomology laboratory, Department of Zoology, IFTM University, Moradabad,244102, India
چکیده [English]

Spodoptera litura (Lepidoptera: Noctuidae) is a polyphagous pest of agricultural crops and causes severe economic loss. In the present study, the biological effect and enzymatic activity of two insect growth regulators, pyriproxyfen and diofenolan was evaluated against S. litura larva. The penultimate instar larvae (0, 24, and 48-hours old) were treated topically at the last three abdominal tergites with sublethal doses (1, 2, and 4µg/µl) of both the juvenile hormonal analogues (JHAs). Several effects were observed such as prolongation in larval-larval and larval-pupal ecdysial duration, larval and pupal mortality, eclosion failure, formation of intermediates, low pupation and reduced adult emergence. Along with these metamorphic catastrophe effects, both JHAs had inhibitory effects for acetylcholinesterase (AChE) but excitatory effects for glutathione –S-transferase enzyme (GST). Whereas, Log IC50 and IC50 values for adult emergence inhibition rate were found to be -0.98 and 0.1 µg/ µl for 24-hours old larvae (Pyriproxyfen); 0.005 and 1.01 µg/ µl for 48-hours old larvae (Pyriproxyfen); -0.085 and 0.82 µg/ µl for 24-hours old larvae (Diofenolan); -0.09 and 1.23 µg/ µl for 48-hours old larvae (Diofenolan). Our result manifests the insecticidal potency of both the IGRs against S. litura.

کلیدواژه‌ها [English]

  • Spodoptera litura
  • pyriproxyfen
  • diofenolan
  • acetylcholinesterase
  • glutathione – S-transferase enzyme

 © 2023 by Author(s), Published by the Entomological Society of Iran

This Work is Licensed under Creative Commons Attribution-Non Commercial 4.0 International Public License

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نامه انجمن حشره شناسی ایران
دوره 43، شماره 4
آذر 1402
صفحه 359-369

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